Antibody Name:	Anti-PAICS Antibody
Antibody SKU:	CAB6450
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 176-425 of human PAICS (NP_001072992.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Rat
Positive Samples:	Jurkat, 293T, SW620, Raji, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 176-425 of human PAICS (NP_001072992.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LEKS WLPQ NCTL VDMK IEFG VDVT TKEI VLAD VIDN DSWR LWPS GDRS QQKD KQSY RDLK EVTP EGLQ MVKK NFEW VAER VELL LKSE SQCR VVVL MGST SDLG HCEK IKKA CGNF GIPC ELRV TSAH KGPD ETLR IKAE YEGD GIPT VFVA VAGR SNGL GPVM SGNT AYPV ISCP PLTP DWGV QDVW SSLR LPSG LGCS TVLS PEGS AQFA AQIF GLSN HLVW SKLR ASIL NTWI SLKQ ADKK IREC NL
Gene ID:	10606
Uniprot:	P22234
Cellular Location:
Calculated MW:	47kDa
Observed MW:	47kDa

Synonyms:

PAICS, ADE2, ADE2H1, AIRC, PAIS

Background:

This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PAICS: 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nucleotide Metabolism - purine; EC 6.3.2.6; Ligase; EC 4.1.1.21; Lyase Chromosomal Location of Human Ortholog: 4q12 Cellular Component: membrane; cytosol Molecular Function:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity; identical protein binding; protein binding; phosphoribosylaminoimidazole carboxylase activity; ATP binding Biological Process: purine ribonucleoside monophosphate biosynthetic process; purine base biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; purine base metabolic process; 'de novo' IMP biosynthetic process
NCBI Summary:	This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P22234
NCBI GenInfo Identifier:	131628
NCBI Gene ID:	10606
NCBI Accession:	P22234.3
UniProt Secondary Accession:	P22234,Q68CQ5, E9PDH9,
UniProt Related Accession:	P22234
Molecular Weight:	425
NCBI Full Name:	Multifunctional protein ADE2
NCBI Synonym Full Names:	phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase
NCBI Official Symbol:	PAICS
NCBI Official Synonym Symbols:	ADE2; AIRC; PAIS; ADE2H1
NCBI Protein Information:	multifunctional protein ADE2; AIR carboxylase; SAICAR synthetase; multifunctional protein ADE2H1
UniProt Protein Name:	Multifunctional protein ADE2
UniProt Synonym Protein Names:	SAICAR synthetase
Protein Family:	Multifunctional protein
UniProt Gene Name:	PAICS
UniProt Entry Name:	PUR6_HUMAN

	Western blot analysis of extracts of various cell lines, using PAICS antibody (CAB6450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat spleen using PAICS antibody (CAB6450) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using PAICS antibody (CAB6450). Blue: DAPI for nuclear staining.