Metabolism Antibodies 1
Anti-PAFAH1B3 Antibody (CAB13037)
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- SKU:
- CAB13037
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-PAFAH1B3 Antibody |
| Antibody SKU: | CAB13037 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-231 of human PAFAH1B3 (NP_002564.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | U-87MG, HeLa, Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-231 of human PAFAH1B3 (NP_002564.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSGE ENPA SKPT PVQD VQGD GRWM SLHH RFVA DSKD KEPE VVFI GDSL VQLM HQCE IWRE LFSP LHAL NFGI GGDG TQHV LWRL ENGE LEHI RPKI VVVW VGTN NHGH TAEQ VTGG IKAI VQLV NERQ PQAR VVVL GLLP RGQH PNPL REKN RQVN ELVR AALA GHPR AHFL DADP GFVH SDGT ISHH DMYD YLHL SRLG YTPV CRAL HSLL LRLL AQDQ GQGA PLLE PAP |
| Gene ID: | 5050 |
| Uniprot: | Q15102 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 25kDa |
| Observed MW: | 25kDa |
| Synonyms: | PAFAH1B3, PAFAHG |
| Background: | This gene encodes an acetylhydrolase that catalyzes the removal of an acetyl group from the glycerol backbone of platelet-activating factor. The encoded enzyme is a subunit of the platelet-activating factor acetylhydrolase isoform 1B complex, which consists of the catalytic beta and gamma subunits and the regulatory alpha subunit. This complex functions in brain development. A translocation between this gene on chromosome 19 and the CDC-like kinase 2 gene on chromosome 1 has been observed, and was associated with mental retardation, ataxia, and atrophy of the brain. Alternatively spliced transcript variants have been described. |
| UniProt Code: | Q15102 |
| NCBI GenInfo Identifier: | 1122219 |
| NCBI Gene ID: | 5050 |
| NCBI Accession: | BAA09706.1 |
| UniProt Secondary Accession: | Q15102,Q53X88, |
| UniProt Related Accession: | Q15102 |
| Molecular Weight: | 25,734 Da |
| NCBI Full Name: | platelet activating factor acetylhydrolase IB gamma-subunit |
| NCBI Synonym Full Names: | platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa) |
| NCBI Official Symbol: | PAFAH1B3 |
| NCBI Official Synonym Symbols: | PAFAHG |
| NCBI Protein Information: | platelet-activating factor acetylhydrolase IB subunit gamma; PAFAH subunit gamma; PAF-AH subunit gamma; PAF-AH 29 kDa subunit; PAF-AH1b alpha 1 subunit; PAF acetylhydrolase 29 kDa subunit; platelet-activating factor acetylhydrolase, isoform Ib, subunit 3 (29kDa) |
| UniProt Protein Name: | Platelet-activating factor acetylhydrolase IB subunit gamma |
| UniProt Synonym Protein Names: | PAF acetylhydrolase 29 kDa subunit; PAF-AH 29 kDa subunit; PAF-AH subunit gamma |
| Protein Family: | PCNA-associated factor |
| UniProt Gene Name: | PAFAH1B3 |
| UniProt Entry Name: | PA1B3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PAFAH1B3 antibody (CAB13037) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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