Immunology Antibodies 2
Anti-PADI4 Antibody (CAB1906)
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- SKU:
- CAB1906
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-PADI4 Antibody |
| Antibody SKU: | CAB1906 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PADI4 (NP_036519.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | BT-474, SKOV3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PADI4 (NP_036519.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAQG TLIR VTPE QPTH AVCV LGTL TQLD ICSS APED CTSF SINA SPGV VVDI AHGP PAKK KSTG SSTW PLDP GVEV TLTM KVAS GSTG DQKV QISY YGPK TPPV KALL YLTG VEIS LCAD ITRT GKVK PTRA VKDQ RTWT WGPC GQGA ILLV NCDR DNLE SSAM DCED DEVL DSED LQDM SLMT LSTK TPKD FFTN HTLV LHVA RSEM DKVR VFQA TRGK LSSK CSVV LGPK WPSH YLMV PGGK HNMD FY |
| Gene ID: | 23569 |
| Uniprot: | Q9UM07 |
| Cellular Location: | Cytoplasm, Cytoplasmic granule, Nucleus |
| Calculated MW: | 74kDa |
| Observed MW: | 74kDa |
| Synonyms: | PADI4, PAD, PAD4, PADI5, PDI4, PDI5 |
| Background: | This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. |
| UniProt Protein Function: | PADI4: Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1. Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA). It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Belongs to the protein arginine deiminase family. |
| UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; Hydrolase; EC 3.5.3.15 Chromosomal Location of Human Ortholog: 1p36.13 Cellular Component: cytoplasm; nucleus Molecular Function:arginine deiminase activity; protein binding; calcium ion binding; protein-arginine deiminase activity Biological Process: chromatin remodeling; nucleosome assembly; transcription, DNA-dependent; regulation of transcription, DNA-dependent; stem cell maintenance; innate immune response; protein modification process; chromatin modification; peptidyl-citrulline biosynthetic process from peptidyl-arginine Disease: Rheumatoid Arthritis |
| NCBI Summary: | This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UM07 |
| NCBI GenInfo Identifier: | 296439260 |
| NCBI Gene ID: | 23569 |
| NCBI Accession: | Q9UM07.2 |
| UniProt Related Accession: | Q9UM07 |
| Molecular Weight: | |
| NCBI Full Name: | Protein-arginine deiminase type-4 |
| NCBI Synonym Full Names: | peptidyl arginine deiminase 4 |
| NCBI Official Symbol: | PADI4 |
| NCBI Official Synonym Symbols: | PAD; PAD4; PDI4; PDI5; PADI5 |
| NCBI Protein Information: | protein-arginine deiminase type-4 |
| UniProt Protein Name: | Protein-arginine deiminase type-4 |
| UniProt Synonym Protein Names: | HL-60 PAD; Peptidylarginine deiminase IV; Protein-arginine deiminase type IV |
| Protein Family: | Lipase |
| UniProt Gene Name: | PADI4 |
| UniProt Entry Name: | PADI4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PADI4 antibody (CAB1906) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded rat lung using PADI4 antibody (CAB1906) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using PADI4 antibody (CAB1906). Blue: DAPI for nuclear staining. |
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