Cell Biology Antibodies 11
Anti-PACSIN2 Antibody (CAB7781)
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- SKU:
- CAB7781
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PACSIN2 Antibody |
| Antibody SKU: | CAB7781 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 240-410 of human PACSIN2 (NP_009160.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | BT-474, HepG2, Mouse lung, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 240-410 of human PACSIN2 (NP_009160.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RLRF FREV LLEV QKHL DLSN VAGY KAIY HDLE QSIR AADA VEDL RWFR ANHG PGMA MNWP QFEE WSAD LNRT LSRR EKKK ATDG VTLT GINQ TGDQ SLPS KPSS TLNV PSNP AQSA QSQS SYNP FEDE DDTG STVS EKDD TKAK NVSS YEKT QSYP TDWS DDES NNPF SST |
| Gene ID: | 11252 |
| Uniprot: | Q9UNF0 |
| Cellular Location: | Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle membrane, Early endosome, Membrane, Peripheral membrane protein, Recycling endosome membrane, caveola, cytoskeleton, ruffle membrane |
| Calculated MW: | 51kDa/55kDa |
| Observed MW: | 56kDa |
| Synonyms: | PACSIN2, SDPII |
| Background: | This gene is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation by regulating tubulin polymerization. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | PACSIN2: May play a role in vesicle formation and transport. Belongs to the PACSIN family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold Chromosomal Location of Human Ortholog: 22q13.2-q13.33 Cellular Component: caveola; cytoplasm; extrinsic to membrane; focal adhesion; intracellular membrane-bound organelle; nucleoplasm; recycling endosome membrane Molecular Function:identical protein binding; protein binding; transporter activity Biological Process: cell projection morphogenesis; regulation of endocytosis |
| NCBI Summary: | This gene is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation by regulating tubulin polymerization. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010] |
| UniProt Code: | Q9UNF0 |
| NCBI GenInfo Identifier: | 22256968 |
| NCBI Gene ID: | 11252 |
| NCBI Accession: | Q9UNF0.2 |
| UniProt Secondary Accession: | Q9UNF0,O95921, Q96HV9, Q9H0D3, Q9NPN1, Q9Y4V2, |
| UniProt Related Accession: | Q9UNF0 |
| Molecular Weight: | 51,353 Da |
| NCBI Full Name: | Protein kinase C and casein kinase substrate in neurons protein 2 |
| NCBI Synonym Full Names: | protein kinase C and casein kinase substrate in neurons 2 |
| NCBI Official Symbol: | PACSIN2 |
| NCBI Official Synonym Symbols: | SDPII |
| NCBI Protein Information: | protein kinase C and casein kinase substrate in neurons protein 2 |
| UniProt Protein Name: | Protein kinase C and casein kinase substrate in neurons protein 2 |
| UniProt Synonym Protein Names: | Syndapin-2; Syndapin-II |
| Protein Family: | Protein kinase C and casein kinase substrate in neurons protein |
| UniProt Gene Name: | PACSIN2 |
| UniProt Entry Name: | PACN2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PACSIN2 antibody (CAB7781) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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