Immunology Antibodies 1

Anti-PACS1 Antibody (CAB12659)

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SKU:
CAB12659
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-PACS1 Antibody
Antibody SKU:CAB12659
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 420-560 of human PACS1 (NP_060496.2).
Application:WB
Recommended Dilution:WB 1:1000 - 1:3000
Reactivity:Human
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 420-560 of human PACS1 (NP_060496.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SKGS LGKD TTSP MELA ALEK IKST WIKN QDDS LTET DTLE ITDQ DMFG DAST SLVV PEKV KTPM KSSK TDLQ GSAS PSKV EGVH TPRQ KRST PLKE RQLS KPLS ERTN SSDS ERSP DLGH STQI PRKV VYDQ LNQI LVSD A
Gene ID:55690
Uniprot:Q6VY07
Cellular Location:Golgi apparatus, trans-Golgi network
Calculated MW:104kDa/109kDa
Observed MW:105kDa
Synonyms:PACS1, MRD17, SHMS
Background:This gene encodes a protein with a putative role in the localization of trans-Golgi network (TGN) membrane proteins. Mouse and rat homologs have been identified and studies of the homologous rat protein indicate a role in directing TGN localization of furin by binding to the protease's phosphorylated cytosolic domain. In addition, the human protein plays a role in HIV-1 Nef-mediated downregulation of cell surface MHC-I molecules to the TGN, thereby enabling HIV-1 to escape immune surveillance.
UniProt Protein Function:PACS-1: Coat protein that is involved in the localization of trans-Golgi network (TGN) membrane proteins that contain acidic cluster sorting motifs. Controls the endosome-to-Golgi trafficking of furin and mannose-6-phosphate receptor by connecting the acidic-cluster-containing cytoplasmic domain of these molecules with the adapter-protein complex-1 (AP-1) of endosomal clathrin- coated membrane pits. Involved in HIV-1 nef-mediated removal of MHC-I from the cell surface to the TGN. Belongs to the PACS family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11q13.1-q13.2

Cellular Component: cytosol

Molecular Function:protein binding

Biological Process: regulation of defense response to virus by virus

Disease: Mental Retardation, Autosomal Dominant 17

NCBI Summary:This gene encodes a protein with a putative role in the localization of trans-Golgi network (TGN) membrane proteins. Mouse and rat homologs have been identified and studies of the homologous rat protein indicate a role in directing TGN localization of furin by binding to the protease's phosphorylated cytosolic domain. In addition, the human protein plays a role in HIV-1 Nef-mediated downregulation of cell surface MHC-I molecules to the TGN, thereby enabling HIV-1 to escape immune surveillance. [provided by RefSeq, Jul 2008]
UniProt Code:Q6VY07
NCBI GenInfo Identifier:52000804
NCBI Gene ID:55690
NCBI Accession:Q6VY07.2
UniProt Secondary Accession:Q6VY07,Q6PJY6, Q6PKB6, Q7Z590, Q7Z5W4, Q8N8K6, Q96MW0 Q9NW92, Q9ULP5,
UniProt Related Accession:Q6VY07
Molecular Weight:105kDa
NCBI Full Name:Phosphofurin acidic cluster sorting protein 1
NCBI Synonym Full Names:phosphofurin acidic cluster sorting protein 1
NCBI Official Symbol:PACS1  
NCBI Official Synonym Symbols:SHMS; MRD17  
NCBI Protein Information:phosphofurin acidic cluster sorting protein 1
UniProt Protein Name:Phosphofurin acidic cluster sorting protein 1
Protein Family:Phosphofurin acidic cluster sorting protein
UniProt Gene Name:PACS1  
Anti-PACS1 Antibody (CAB12659)Western blot analysis of extracts of 293T cells, using PACS1 antibody (CAB12659) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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