Name: Anti-PACRG Antibody (CAB15956)
Brand: Cell Biology Antibodies 6
SKU: CAB15956
Availability: OutOfStock

Antibody Name:	Anti-PACRG Antibody
Antibody SKU:	CAB15956
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 150-250 of human PACRG (NP_001073847.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, A-549, BxPC-3, Raji, Mouse testis, Mouse lung, Rat testis

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 150-250 of human PACRG (NP_001073847.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IIPI KNAL NLRN RQVI CVTL KVLQ HLVV SAEM VGKA LVPY YRQI LPVL NIFK NMNV NSGD GIDY SQQK RENI GDLI QETL EAFE RYGG ENAF INIK YVVP T
Gene ID:	135138
Uniprot:	Q96M98
Cellular Location:
Calculated MW:	29kDa/33kDa
Observed MW:	35kDa

Synonyms:

PACRG, GLUP, HAK005771, PARK2CRG

Background:

This gene encodes a protein that is conserved across metazoans. In vertebrates, this gene is linked in a head-to-head arrangement with the adjacent parkin gene, which is associated with autosomal recessive juvenile Parkinson's disease. These genes are co-regulated in various tissues and they share a bi-directional promoter. Both genes are associated with susceptibility to leprosy. The parkin co-regulated gene protein forms a large molecular complex with chaperones, including heat shock proteins 70 and 90, and chaperonin components. This protein is also a component of Lewy bodies in Parkinson's disease patients, and it suppresses unfolded Pael receptor-induced neuronal cell death. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PACRG: Suppresses cell death induced by accumulation of unfolded Pael receptor (Pael-R, a substrate of Parkin). Facilitates the formation of inclusions consisting of Pael-R, molecular chaperones, protein degradation molecules and itself when proteasome is inhibited. May play an important role in the formation of Lewy bodies and protection of dopaminergic neurons against Parkinson disease. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Mitochondrial Chromosomal Location of Human Ortholog: 6q26 Cellular Component: cytosol; neuron projection; nucleus; vesicle Molecular Function:actin binding; alpha-tubulin binding; beta-tubulin binding; chaperone binding; G-protein-coupled receptor binding; heat shock protein binding; Hsp70 protein binding; Hsp90 protein binding; ubiquitin protein ligase binding Disease: Leprosy, Susceptibility To, 2
NCBI Summary:	This gene encodes a protein that is conserved across metazoans. In vertebrates, this gene is linked in a head-to-head arrangement with the adjacent parkin gene, which is associated with autosomal recessive juvenile Parkinson's disease. These genes are co-regulated in various tissues and they share a bi-directional promoter. Both genes are associated with susceptibility to leprosy. The parkin co-regulated gene protein forms a large molecular complex with chaperones, including heat shock proteins 70 and 90, and chaperonin components. This protein is also a component of Lewy bodies in Parkinson's disease patients, and it suppresses unfolded Pael receptor-induced neuronal cell death. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q96M98
NCBI GenInfo Identifier:	77416872
NCBI Gene ID:	135138
NCBI Accession:	Q96M98.2
UniProt Secondary Accession:	Q96M98,Q6IMB8, Q8IZM1, Q8NHP5, Q9H1V9, E1P5B5,
UniProt Related Accession:	Q96M98
Molecular Weight:	29,312 Da
NCBI Full Name:	Parkin coregulated gene protein
NCBI Synonym Full Names:	PARK2 coregulated
NCBI Official Symbol:	PACRG
NCBI Official Synonym Symbols:	GLUP; PARK2CRG; HAK005771
NCBI Protein Information:	parkin coregulated gene protein
UniProt Protein Name:	Parkin coregulated gene protein
UniProt Synonym Protein Names:	Molecular chaperone/chaperonin-binding protein; PARK2 coregulated gene protein
Protein Family:	PACRG-like protein
UniProt Gene Name:	PACRG
UniProt Entry Name:	PACRG_HUMAN

Western blot analysis of extracts of various cell lines, using PACRG antibody (CAB15956) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

ELISA Kits

Human PACRG / Parkin coregulated gene protein ELISA Kit

Human Parkin coregulated gene protein (PACRG) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)