Cell Cycle Antibodies 2
Anti-p60 CAF1 Antibody (CAB5073)
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- SKU:
- CAB5073
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- CAF-1
- Synonyms:
- CAF-IP60
- Synonyms:
- CAF1
- Synonyms:
- CAF1A
- Synonyms:
- CAF1P60
- Synonyms:
- MPHOSPH7
- Synonyms:
- MPP7
- Research Area:
- Cell Cycle
Description
| Product Name: | p60 CAF1 Rabbit mAb |
| Product Code: | CAB5073 |
| Size: | 20uL, 50uL, 100uL |
| Synonyms: | CAF-1, CAF-IP60, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7 |
| Applications: | WB, IHC |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human p60 CAF1 |
| Applications: | WB, IHC |
| Recommended Dilutions: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | HeLa, 293T, Jurkat |
| Immunogen: | A synthesized peptide derived from human p60 CAF1 |
| Purification Method: | Affinity purification |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 8208 |
| Uniprot: | Q13112 |
| Calculated MW: | 70kDa |
| Observed MW: | 70KDa |
| UniProt Protein Function: | Function: Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. Ref.6 |
| UniProt Protein Details: | Subunit structure: Subunit of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. In G2 and S phase also monomeric CHAF1B is detected. Subcellular location: Nucleus. Cytoplasm. Note: DNA replication foci. Cytoplasmic in M phase. Ref.7 Developmental stage: Active complex is found in G1, S and G2 phases. Post-translational modification: Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases. Ref.5 Ref.7 Sequence similarities: Belongs to the WD repeat HIR1 family.Contains 7 WD repeats. |
| NCBI Summary: | Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13112 |
| NCBI GenInfo Identifier: | 3121829 |
| NCBI Gene ID: | 8208 |
| NCBI Accession: | Q13112.1 |
| UniProt Secondary Accession: | Q13112,Q99548, |
| UniProt Related Accession: | Q13112 |
| Molecular Weight: | 61,493 Da |
| NCBI Full Name: | Chromatin assembly factor 1 subunit B |
| NCBI Synonym Full Names: | chromatin assembly factor 1, subunit B (p60) |
| NCBI Official Symbol: | CHAF1B |
| NCBI Official Synonym Symbols: | CAF1; MPP7; CAF-1; CAF1A; CAF1P60; CAF-IP60; MPHOSPH7 |
| NCBI Protein Information: | chromatin assembly factor 1 subunit B; CAF-I p60; CAF-1 subunit B; CAF-I 60 kDa subunit; M-phase phosphoprotein 7; chromatin assembly factor I p60 subunit; human chromatin assembly factor-I p60 subunit |
| UniProt Protein Name: | Chromatin assembly factor 1 subunit B |
| UniProt Synonym Protein Names: | Chromatin assembly factor I p60 subunit; CAF-I 60 kDa subunit; CAF-I p60; M-phase phosphoprotein 7 |
| UniProt Gene Name: | CHAF1B |
| UniProt Entry Name: | CAF1B_HUMAN |
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