KO Validated Antibodies 1

Anti-p53 Antibody (CAB5761)[KO Validated]

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SKU:
CAB5761
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Applications:
IP
Applications:
ChIP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-p53 Antibody [KO Validated]
Antibody SKU:CAB5761
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP ChIP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human p53 (NP_000537.3).
Application:WB IHC IP ChIP
Recommended Dilution:WB 1:3000 - 1:6000 IHC 1:50 - 1:200 IP 1:20 - 1:50 ChIP 1:20 - 1:50
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, BT-474, MCF7, Mouse spleen, Mouse testis, Mouse kidney, Rat kidney
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human p53 (NP_000537.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EPPE AGSE YTTI HYKY MCNS SCMG GMNR RPIL TIIT LEDS SGNL LGRD SFEV RVCA CPGR DRRT EEEN FRKK EVLC PELP PGSA KRAL PTCT SASP PQKK KPLD GEYF TLKI RGRK RFEM FREL NEAL ELKD AHAT EESG DSRA HSSY LKTK KGQS TSRH KKTM VKKV GPDS D
Gene ID:22059
Uniprot:P02340
Cellular Location:Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Calculated MW:43kDa
Observed MW:53kDa
Synonyms:TP53, BCC7, LFS1, P53, TRP53, Trp53, bbl, bfy, bhy, p44, p53, Tp53
Background:This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
UniProt Protein Function:p53: a transcription factor and major tumor suppressor that plays a major role in regulating cellular responses to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. More than 50 percent of human tumors contain a mutation or deletion of the TP53 gene. p53 is modified post-translationally at multiple sites. DNA damage induces phosphorylation of p53 at S15, S20 and S37, reducing its interaction with the oncoprotein MDM2. MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation. Phosphorylated by many kinases including Chk2 and Chk1 at S20, enhancing its tetramerization, stability and activity. The phosphorylation by CAK at S392 is increased in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53. Phosphorylation of p53 at S46 regulates the ability of p53 to induce apoptosis. The acetylation of p53 appears to play a positive role in the accumulation of p53 during the stress response. Following DNA damage, p53 becomes acetylated at K382, enhancing its binding to DNA. Deacetylation of p53 can occur through interaction with SIRT1, a deacetylase that may be involved in cellular aging and the DNA damage response. p53 regulates the transcription of a set of genes encoding endosomal proteins that regulate endosomal functions. These include STEAP3 and CHMP4C, which enhance exosome production, and CAV1 and CHMP4C, which produce a more rapid endosomal clearance of the EGFR from the plasma membrane. DNA damage regulates a p53-mediated secretory pathway, increasing the secretion of some proteins such as Hsp90, SERPINE1, SERPINB5, NKEF-A, and CyPA, and inhibiting the secretion of others including CTSL and IGFBP-2. Two alternatively spliced human isoforms have been reported. Isoform 2 is expressed in quiescent lymphocytes. Seems to be non-functional. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
UniProt Protein Details:

Protein type:Tumor suppressor; Transcription factor; DNA-binding; Activator; Motility/polarity/chemotaxis; Nuclear receptor co-regulator

Cellular Component: PML body; transcription factor TFIID complex; nuclear matrix; protein complex; mitochondrion; endoplasmic reticulum; replication fork; cytosol; nucleoplasm; nuclear body; transcription factor complex; mitochondrial matrix; nuclear chromatin; cytoplasm; nucleolus; intracellular; chromatin; nucleus

Molecular Function:identical protein binding; protease binding; protein phosphatase 2A binding; metal ion binding; protein phosphatase binding; transcription factor binding; histone acetyltransferase binding; enzyme binding; sequence-specific DNA binding; double-stranded DNA binding; transcription factor activity; ATP binding; protein C-terminus binding; p53 binding; protein N-terminus binding; receptor tyrosine kinase binding; protein kinase binding; protein binding; histone deacetylase regulator activity; copper ion binding; DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; chaperone binding; damaged DNA binding; chromatin binding

Biological Process: central nervous system development; positive regulation of apoptosis; regulation of cell cycle; positive regulation of leukocyte migration; positive regulation of transcription, DNA-dependent; multicellular organismal development; T cell differentiation in the thymus; programmed cell death; gastrulation; determination of adult life span; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; cellular response to glucose starvation; protein localization; negative regulation of neuroblast proliferation; transforming growth factor beta receptor signaling pathway; regulation of neuron apoptosis; cerebellum development; protein complex assembly; negative regulation of mitotic cell cycle; cell cycle arrest; ER overload response; response to X-ray; response to UV; response to drug; release of cytochrome c from mitochondria; somitogenesis; transcription, DNA-dependent; chromatin assembly; positive regulation of cell cycle; cell aging; circadian behavior; rRNA transcription; regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of DNA replication; negative regulation of fibroblast proliferation; regulation of intracellular pH; embryonic organ development; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; negative regulation of transcription, DNA-dependent; regulation of tissue remodeling; negative regulation of apoptosis; transcription from RNA polymerase II promoter; G1 DNA damage checkpoint; DNA damage response, signal transduction by p53 class mediator; negative regulation of smooth muscle cell proliferation; apoptosis; negative regulation of transcription from RNA polymerase II promoter; chromosome organization and biogenesis; response to salt stress; entrainment of circadian clock by photoperiod; embryonic development ending in birth or egg hatching; positive regulation of protein oligomerization; negative regulation of cell proliferation; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; positive regulation of histone deacetylation; regulation of transcription, DNA-dependent; T cell proliferation during immune response; regulation of catalytic activity; double-strand break repair; positive regulation of neuron apoptosis; response to gamma radiation; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; protein tetramerization; mitochondrial DNA repair; negative regulation of proteolysis; in utero embryonic development; B cell lineage commitment; multicellular organism growth; cell cycle; regulation of cell proliferation; T cell lineage commitment; neuron apoptosis; nucleotide-excision repair; protein import into nucleus, translocation; DNA strand renaturation; negative regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; response to DNA damage stimulus

NCBI Summary:This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mice deficient for this gene are developmentally normal but are susceptible to spontaneous tumors. Evidence to date shows that this gene contains one promoter, in contrast to alternative promoters of the human gene, and transcribes a few of splice variants which encode different isoforms, although the biological validity or the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:P02340
NCBI GenInfo Identifier:172047304
NCBI Gene ID:22059
NCBI Accession:P02340.3
UniProt Secondary Accession:P02340,Q9QUP3,
UniProt Related Accession:P02340
Molecular Weight:393
NCBI Full Name:Cellular tumor antigen p53
NCBI Synonym Full Names:transformation related protein 53
NCBI Official Symbol:Trp53
NCBI Official Synonym Symbols:bbl; bfy; bhy; p44; p53; Tp53
NCBI Protein Information:cellular tumor antigen p53; tumor supressor p53; tumor suppressor p53; p53 cellular tumor antigen
UniProt Protein Name:Cellular tumor antigen p53
UniProt Synonym Protein Names:Tumor suppressor p53
Protein Family:TP53-regulating kinase
UniProt Gene Name:Tp53
UniProt Entry Name:P53_MOUSE
Anti-p53 Antibody [KO Validated] (CAB5761)Western blot analysis of extracts of various cell lines, using p53 antibody (CAB5761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-p53 Antibody [KO Validated] (CAB5761)Western blot analysis of extracts from normal (control) and p53 knockout (KO) 293T cells, using p53 antibody (CAB5761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-p53 Antibody [KO Validated] (CAB5761)Immunohistochemistry of paraffin-embedded Human oophoroma using p53 antibody (CAB5761) at dilution of 1:200 (40x lens).
Anti-p53 Antibody [KO Validated] (CAB5761)Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug p53 antibody (CAB5761). Western blot was performed from the immunoprecipitate using p53 antibody (CAB5761) at a dilition of 1:500.
Anti-p53 Antibody [KO Validated] (CAB5761)Chromatin immunoprecipitation analysis of extracts of 293T cells, using p53 antibody (CAB5761) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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