Name: Anti-P4HTM Antibody (CAB6143)
Brand: Cell Biology Antibodies 10
SKU: CAB6143
Availability: OutOfStock

Antibody Name:	Anti-P4HTM Antibody
Antibody SKU:	CAB6143
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 294-563 of human P4HTM (NP_808807.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	MCF7, Jurkat, 293T, U-87MG, HeLa, Mouse heart, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 294-563 of human P4HTM (NP_808807.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RQRV LRLT RLSP EIVE LSEP LQVV RYGE GGHY HAHV DSGP VYPE TICS HTKL VANE SVPF ETSC RQVS PNWG LPSI LRPG TPMT QAQP CTVG VPLG MGPG DHWV IPVS PWEH PQLG TCSV PPLP YSYM TVLF YLNN VTGG GETV FPVA DNRT YDEM SLIQ DDVD LRDT RRHC DKGN LRVK PQQG TAVF WYNY LPDG QGWV GDVD DYSL HGGC LVTR GTKW IANN WINV DPSR ARQA LFQQ EMAR LARE GGTD SQPE WALD RAYR DARV EL
Gene ID:	54681
Uniprot:	Q9NXG6
Cellular Location:	Endoplasmic reticulum membrane, Single-pass type II membrane protein
Calculated MW:	47kDa/56kDa/63kDa
Observed MW:	55kDa

Synonyms:	P4HTM, EGLN4, HIFPH4, P4H-TM, PH-4, PH4, PHD4
Background:	The product of this gene belongs to the family of prolyl 4-hydroxylases. This protein is a prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. It plays a role in adaptation to hypoxia and may be related to cellular oxygen sensing. Alternatively spliced variants encoding different isoforms have been identified.

UniProt Protein Function:	PH4: Catalyzes the post-translational formation of 4- hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Oxidoreductase; EC 1.14.11.-; Membrane protein, integral Chromosomal Location of Human Ortholog: 3p21.31\|3p21.3 Cellular Component: endoplasmic reticulum membrane; integral to membrane Molecular Function:L-ascorbic acid binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; calcium ion binding
NCBI Summary:	The product of this gene belongs to the family of prolyl 4-hydroxylases. This protein is a prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. It plays a role in adaptation to hypoxia and may be related to cellular oxygen sensing. Alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NXG6
NCBI GenInfo Identifier:	32129516
NCBI Gene ID:	54681
NCBI Accession:	Q9NXG6.2
UniProt Secondary Accession:	Q9NXG6,Q6PAG6, Q8TCJ9, Q8WV55, Q96F22, Q9BW77,
UniProt Related Accession:	Q9NXG6
Molecular Weight:	502
NCBI Full Name:	Transmembrane prolyl 4-hydroxylase
NCBI Synonym Full Names:	prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum)
NCBI Official Symbol:	P4HTM
NCBI Official Synonym Symbols:	PH4; PH-4; PHD4; EGLN4; HIFPH4; P4H-TM
NCBI Protein Information:	transmembrane prolyl 4-hydroxylase; HPH-4; HIF-PH4; proline 4-hydroxylase; HIF-prolyl hydroxylase 4; P4H with transmembrane domain; Prolyl hydroxlase domain-containing 4; hypoxia inducible factor prolyl 4 hydroxylase; hypoxia-inducible factor prolyl 4-hydroxylase; hypoxia-inducible factor prolyl hydroxylase 4
UniProt Protein Name:	Transmembrane prolyl 4-hydroxylase
UniProt Synonym Protein Names:	Hypoxia-inducible factor prolyl hydroxylase 4; HIF-PH4; HIF-prolyl hydroxylase 4; HPH-4
Protein Family:	Transmembrane prolyl 4-hydroxylase
UniProt Gene Name:	P4HTM
UniProt Entry Name:	P4HTM_HUMAN

Western blot analysis of extracts of various cell lines, using P4HTM antibody (CAB6143) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s.

Antibodies

Anti-P4HTM Antibody (CAB13593)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)