Anti-p150 CAF1 Antibody (CAB8725)
- SKU:
- CAB8725
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- CAF-1
- Synonyms:
- CAF1
- Synonyms:
- CAF1B
- Synonyms:
- CAF1P150
- Synonyms:
- P150
- Research Area:
- Cell Cycle
Description
| Product Name: | p150 CAF1 Rabbit mAb |
| Product Code: | CAB8725 |
| Size: | 20uL, 50uL, 100uL |
| Synonyms: | CAF-1, CAF1, CAF1B, CAF1P150, P150 |
| Applications: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human p150 CAF1 |
| Applications: | WB |
| Recommended Dilutions: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | HeLa, Jurkat, SH-SY5Y |
| Immunogen: | A synthesized peptide derived from human p150 CAF1 |
| Purification Method: | Affinity purification |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 10036 |
| Uniprot: | Q13111 |
| Calculated MW: | 150kDa |
| Observed MW: | 150kDa |
| UniProt Protein Function: | CAF-1A: Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci. The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D- coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene. Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. Part of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. CHAF1A binds directly to PCNA and to CBX1. Binds MBD1. Interacts directly with CBX5 via the PxVxL motif. During DNA replication, it forms a S phase- specific complex that facilitates DNA methylation and histone H3 'Lys-9' methylation during replication-coupled chromatin assembly and is at least composed of the CHAF1A, MBD1 and SETDB1. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B. Interacts with CBX5. Belongs to the CHAF1A family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA replication Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: protein complex; nuclear chromatin Molecular Function:identical protein binding; protein binding; unfolded protein binding; chromatin binding Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; chromatin assembly; protein complex assembly; DNA repair; cell cycle; DNA replication; DNA replication-dependent nucleosome assembly |
| NCBI Summary: | Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q13111 |
| NCBI GenInfo Identifier: | 229462842 |
| NCBI Gene ID: | 10036 |
| NCBI Accession: | Q13111.2 |
| UniProt Secondary Accession: | Q13111,Q6NXG5, Q7Z7K3, Q9UJY8, |
| UniProt Related Accession: | Q13111 |
| Molecular Weight: | 956 |
| NCBI Full Name: | Chromatin assembly factor 1 subunit A |
| NCBI Synonym Full Names: | chromatin assembly factor 1, subunit A (p150) |
| NCBI Official Symbol: | CHAF1A |
| NCBI Official Synonym Symbols: | CAF1; P150; CAF-1; CAF1B; CAF1P150 |
| NCBI Protein Information: | chromatin assembly factor 1 subunit A; hp150; CAF-I p150; CAF-1 subunit A; CAF-I 150 kDa subunit; chromatin assembly factor I (150 kDa); chromatin assembly factor I p150 subunit |
| UniProt Protein Name: | Chromatin assembly factor 1 subunit A |
| UniProt Synonym Protein Names: | Chromatin assembly factor I p150 subunit; CAF-I 150 kDa subunit; CAF-I p150; hp150 |
| Protein Family: | Chromatin assembly factor |
| UniProt Gene Name: | CHAF1A |
| UniProt Entry Name: | CAF1A_HUMAN |
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