Signal Transduction Antibodies 1
Anti-P Glycoprotein Antibody (CAB11758)
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- SKU:
- CAB11758
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-P Glycoprotein Antibody |
| Antibody SKU: | CAB11758 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 600-700 of human P Glycoprotein (NP_000918.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, Mouse heart |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 600-700 of human P Glycoprotein (NP_000918.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GFDD GVIV EKGN HDEL MKEK GIYF KLVT MQTA GNEV ELEN AADE SKSE IDAL EMSS NDSR SSLI RKRS TRRS VRGS QAQD RKLS TKEA LDES IPPV SFWR I |
| Gene ID: | 5243 |
| Uniprot: | P08183 |
| Cellular Location: | Cell membrane, Multi-pass membrane protein |
| Calculated MW: | 134kDa/141kDa |
| Observed MW: | 180kDa |
| Synonyms: | ABC20, CD243, CLCS, GP170, MDR1, P-GP, PGY1, P Glycoprotein, ABCB1 |
| Background: | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants. |
| UniProt Protein Function: | ABCB1: Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. Interacts with PSMB8. |
| UniProt Protein Details: | Protein type:Hydrolase; Cell adhesion; Transporter; Membrane protein, integral; Membrane protein, multi-pass; EC 3.6.3.44; Transporter, ABC family Chromosomal Location of Human Ortholog: 7q21.12 Cellular Component: cell surface; membrane; plasma membrane; integral to membrane Molecular Function:protein binding; ATPase activity, coupled to transmembrane movement of substances; transporter activity; xenobiotic-transporting ATPase activity; ATP binding Biological Process: response to drug; transport; multidrug transport; xenobiotic transport; G2/M transition of mitotic cell cycle; transmembrane transport Disease: Colchicine Resistance; Inflammatory Bowel Disease 13 |
| NCBI Summary: | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P08183 |
| NCBI GenInfo Identifier: | 238054374 |
| NCBI Gene ID: | 5243 |
| NCBI Accession: | P08183.3 |
| UniProt Secondary Accession: | P08183,Q12755, Q14812, A8K294, B5AK60, |
| UniProt Related Accession: | P08183 |
| Molecular Weight: | 134,032 Da |
| NCBI Full Name: | Multidrug resistance protein 1 |
| NCBI Synonym Full Names: | ATP-binding cassette, sub-family B (MDR/TAP), member 1 |
| NCBI Official Symbol: | ABCB1 |
| NCBI Official Synonym Symbols: | CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170 |
| NCBI Protein Information: | multidrug resistance protein 1; P-glycoprotein 1; colchicin sensitivity; doxorubicin resistance |
| UniProt Protein Name: | Multidrug resistance protein 1 |
| UniProt Synonym Protein Names: | ATP-binding cassette sub-family B member 1; P-glycoprotein 1; CD_antigen: CD243 |
| Protein Family: | ABC transporter B family |
| UniProt Gene Name: | ABCB1 |
| UniProt Entry Name: | MDR1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using P Glycoprotein antibody (CAB11758) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain endothelial cells using P Glycoprotein antibody (CAB11758) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung cancer using P Glycoprotein antibody (CAB11758) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of NIH/3T3 cells using P Glycoprotein antibody (CAB11758) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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