Cell Biology Antibodies 9
Anti-OTX2 Antibody (CAB5475)
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- SKU:
- CAB5475
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-OTX2 Antibody |
| Antibody SKU: | CAB5475 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 140-280 of human OTX2 (NP_068374.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse liver, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 140-280 of human OTX2 (NP_068374.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TPPS STSV PTIA SSSA PVSI WSPA SISP LSDP LSTS SSCM QRSY PMTY TQAS GYSQ GYAG STSY FGGM DCGS YLTP MHHQ LPGP GATL SPMG TNAV TSHL NQSP ASLS TQGY GASS LGFN STTD CLDY KDQT ASWK LNFN A |
| Gene ID: | 5015 |
| Uniprot: | P32243 |
| Cellular Location: | Nucleus |
| Calculated MW: | 31kDa/32kDa |
| Observed MW: | 32kDa |
| Synonyms: | OTX2, CPHD6, MCOPS5 |
| Background: | This gene encodes a member of the bicoid subfamily of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and plays a role in brain, craniofacial, and sensory organ development. The encoded protein also influences the proliferation and differentiation of dopaminergic neuronal progenitor cells during mitosis. Mutations in this gene cause syndromic microphthalmia 5 (MCOPS5) and combined pituitary hormone deficiency 6 (CPHD6). This gene is also suspected of having an oncogenic role in medulloblastoma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Pseudogenes of this gene are known to exist on chromosomes two and nine. |
| UniProt Protein Function: | OTX2: Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'. Expressed in brain. Belongs to the paired homeobox family. Bicoid subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 14q22.3 Cellular Component: growth cone; nucleus; protein complex Molecular Function:eukaryotic initiation factor 4E binding; protein binding Biological Process: axon guidance; forebrain development; midbrain development; positive regulation of embryonic development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein complex assembly; regulation of fibroblast growth factor receptor signaling pathway; regulation of smoothened signaling pathway Disease: Microphthalmia, Syndromic 5; Pituitary Hormone Deficiency, Combined, 6 |
| NCBI Summary: | This gene encodes a member of the bicoid subfamily of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and plays a role in brain, craniofacial, and sensory organ development. The encoded protein also influences the proliferation and differentiation of dopaminergic neuronal progenitor cells during mitosis. Mutations in this gene cause syndromic microphthalmia 5 (MCOPS5) and combined pituitary hormone deficiency 6 (CPHD6). This gene is also suspected of having an oncogenic role in medulloblastoma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Pseudogenes of this gene are known to exist on chromosomes two and nine. [provided by RefSeq, Jul 2012] |
| UniProt Code: | P32243 |
| NCBI GenInfo Identifier: | 417427 |
| NCBI Gene ID: | 5015 |
| NCBI Accession: | P32243.1 |
| UniProt Secondary Accession: | P32243,Q6GTV3, Q9HAW3, Q9P2R1, B2RAN5, |
| UniProt Related Accession: | P32243 |
| Molecular Weight: | 32,406 Da |
| NCBI Full Name: | Homeobox protein OTX2 |
| NCBI Synonym Full Names: | orthodenticle homeobox 2 |
| NCBI Official Symbol: | OTX2 |
| NCBI Official Synonym Symbols: | CPHD6; MCOPS5 |
| NCBI Protein Information: | homeobox protein OTX2 |
| UniProt Protein Name: | Homeobox protein OTX2 |
| UniProt Synonym Protein Names: | Orthodenticle homolog 2 |
| Protein Family: | Homeobox protein |
| UniProt Gene Name: | OTX2 |
 | Western blot analysis of extracts of various cell lines, using OTX2 antibody (CAB5475) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s. |
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