Immunology Antibodies 1

Anti-OTUB1 Antibody (CAB11656)

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SKU:
CAB11656
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-OTUB1 Antibody
Antibody SKU:CAB11656
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human OTUB1 (NP_060140.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, A375, HT-29, 293T, Mouse brain, Mouse heart, Mouse kidney, Mouse testis, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human OTUB1 (NP_060140.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAE EPQQ QKQE PLGS DSEG VNCL AYDE AIMA QQDR IQQE IAVQ NPLV SERL ELSV LYKE YAED DNIY QQKI KDLH KKYS YIRK TRPD GNCF YRAF GFSH LEAL LDDS KELQ RFKA VSAK SKED LVSQ GFTE FTIE DFHN TFMD LIEQ VEKQ TSVA DLLA SFND QSTS DYLV VYLR LLTS GYLQ RESK FFEH FIEG GRTV KEFC QQEV EPMC KESD HIHI
Gene ID:55611
Uniprot:Q96FW1
Cellular Location:Cytoplasm
Calculated MW:31kDa/35kDa
Observed MW:37kDa
Synonyms:OTUB1, HSPC263, OTB1, OTU1
Background:The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:OTUB1: a protease that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occur when T-cell are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Two alternatively spliced isoforms are known.
UniProt Protein Details:

Protein type:Ubiquitin-specific protease; EC 3.4.19.12; Protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: cytoplasm; nucleus

Molecular Function:NEDD8-specific protease activity; protein binding; ubiquitin binding; ubiquitin protein ligase binding; ubiquitin-specific protease activity

Biological Process: adaptive immune response; DNA repair; response to DNA damage stimulus

NCBI Summary:The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
UniProt Code:Q96FW1
NCBI GenInfo Identifier:44888286
NCBI Gene ID:55611
NCBI Accession:Q96FW1.2
UniProt Secondary Accession:Q96FW1,Q32Q78, Q96II3, Q9NXQ4, Q9P0B8,
UniProt Related Accession:Q96FW1
Molecular Weight:35,285 Da
NCBI Full Name:Ubiquitin thioesterase OTUB1
NCBI Synonym Full Names:OTU deubiquitinase, ubiquitin aldehyde binding 1
NCBI Official Symbol:OTUB1  
NCBI Official Synonym Symbols:OTB1; OTU1; HSPC263  
NCBI Protein Information:ubiquitin thioesterase OTUB1
UniProt Protein Name:Ubiquitin thioesterase OTUB1
UniProt Synonym Protein Names:Deubiquitinating enzyme OTUB1; OTU domain-containing ubiquitin aldehyde-binding protein 1; Otubain-1; hOTU1; Ubiquitin-specific-processing protease OTUB1
Protein Family:Ubiquitin thioesterase
UniProt Gene Name:OTUB1  
UniProt Entry Name:OTUB1_HUMAN
Anti-OTUB1 Antibody (CAB11656)Western blot analysis of extracts of various cell lines, using OTUB1 antibody (CAB11656) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
Anti-OTUB1 Antibody (CAB11656)Immunofluorescence analysis of H9C2 cells using OTUB1 antibody (CAB11656) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-OTUB1 Antibody (CAB11656)Immunofluorescence analysis of U2OS cells using OTUB1 antibody (CAB11656) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-OTUB1 Antibody (CAB11656)Immunofluorescence analysis of L929 cells using OTUB1 antibody (CAB11656) at dilution of 1:100. Blue: DAPI for nuclear staining.
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system_update_altDatasheetHuman OTUB1/OTB1 Recombinant Protein Ubiquitin thioesterase OTUB1, also known as Deubiquitinating enzyme OTUB1, OTU domain-containing ubiquitin aldehyde-binding protei