Name: Anti-OSTM1 Antibody (CAB11685)
Brand: Cell Biology Antibodies 2
SKU: CAB11685
Availability: OutOfStock

Antibody Name:	Anti-OSTM1 Antibody
Antibody SKU:	CAB11685
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 32-284 of human OSTM1 (NP_054747.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, 293T, A-549, Raji, B cells, Mouse brain, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 32-284 of human OSTM1 (NP_054747.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ALPF GSSP HRVF HDLL SEQQ LLEV EDLS LSLL QGGG LGPL SLPP DLPD LDPE CREL LLDF ANSS AELT GCLV RSAR PVRL CQTC YPLF QQVV SKMD NISR AAGN TSES QSCA RSLL MADR MQIV VILS EFFN TTWQ EANC ANCL TNNS EELS NSTV YFLN LFNH TLTC FEHN LQGN AHSL LQTK NYSE VCKN CREA YKTL SSLY SEMQ KMNE LENK AEPG THLC IDVE DAMN ITRK LWSR TFNC SVPC SDTV P
Gene ID:	28962
Uniprot:	Q86WC4
Cellular Location:	Lysosome membrane, Single-pass type I membrane protein
Calculated MW:	37kDa
Observed MW:	30-37kDa

Synonyms:

OSTM1, GIPN, GL, HSPC019, OPTB5

Background:

This gene encodes a protein that may be involved in the degradation of G proteins via the ubiquitin-dependent proteasome pathway. The encoded protein binds to members of subfamily A of the regulator of the G-protein signaling (RGS) family through an N-terminal leucine-rich region. This protein also has a central RING finger-like domain and E3 ubiquitin ligase activity. This protein is highly conserved from flies to humans. Defects in this gene may cause the autosomal recessive, infantile malignant form of osteopetrosis.

UniProt Protein Function:	OSTM1: Required for osteoclast and melanocyte maturation and function. Defects in OSTM1 are the cause of osteopetrosis autosomal recessive type 5 (OPTB5); also called infantile malignant osteopetrosis 3. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. OPTB5 patients manifest primary central nervous system involvement in addition to the classical stigmata of severe bone sclerosis, growth failure, anemia, thrombocytopenia and visual impairment with optic atrophy.
UniProt Protein Details:	Protein type:EC 6.3.2.-; Ligase; Membrane protein, integral; Ubiquitin conjugating system; Ubiquitin ligase Chromosomal Location of Human Ortholog: 6q21 Cellular Component: lysosomal membrane Disease: Osteopetrosis, Autosomal Recessive 5
NCBI Summary:	This gene encodes a protein that may be involved in the degradation of G proteins via the ubiquitin-dependent proteasome pathway. The encoded protein binds to members of subfamily A of the regulator of the G-protein signaling (RGS) family through an N-terminal leucine-rich region. This protein also has a central RING finger-like domain and E3 ubiquitin ligase activity. This protein is highly conserved from flies to humans. Defects in this gene may cause the autosomal recessive, infantile malignant form of osteopetrosis. [provided by RefSeq, Jul 2008]
UniProt Code:	Q86WC4
NCBI GenInfo Identifier:	51316434
NCBI Gene ID:	28962
NCBI Accession:	Q86WC4.1
UniProt Secondary Accession:	Q86WC4,Q5R391, Q6PCA7, Q7RTW6, Q8NC29, Q8TC82, Q9Y2S9 E1P5E3,
UniProt Related Accession:	Q86WC4
Molecular Weight:	37kDa
NCBI Full Name:	Osteopetrosis-associated transmembrane protein 1
NCBI Synonym Full Names:	osteopetrosis associated transmembrane protein 1
NCBI Official Symbol:	OSTM1
NCBI Official Synonym Symbols:	GL; GIPN; OPTB5; HSPC019
NCBI Protein Information:	osteopetrosis-associated transmembrane protein 1
UniProt Protein Name:	Osteopetrosis-associated transmembrane protein 1
UniProt Synonym Protein Names:	Chloride channel 7 beta subunit
Protein Family:	Osteopetrosis-associated transmembrane protein
UniProt Gene Name:	OSTM1

Western blot analysis of extracts of various cell lines, using OSTM1 antibody (CAB11685) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

OSTM1 Antibody (PACO11028)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)