Anti-Osteocalcin (BGLAP) Antibody (CAB20800)
- SKU:
- CAB20800
- Product Type:
- Antibody
- Antibody Type:
- Secondary Antibody
- Host Species:
- Donkey
- Isotype:
- Fluorescein conjugated IgG
Description
| Antibody Name: | Anti-Osteocalcin (BGLAP) Antibody (CAB20800) |
| Antibody SKU: | CAB20800 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human BGLAP. |
| Application: | Western blotting |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, U-87MG, Mouse fat, Rat lung, Mouse lung, Rat brain, Rat spleen |
| Immunogen: | Recombinant protein of human BGLAP. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Cellular Location: | Secreted |
| Calculated MW: | 11kDa |
| Observed MW: | 11kDa |
| Synonyms: | BGLAP, BGP, OC, OCN, osteocalcin |
| Background: | This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. |
 | Western blot analysis of extracts of various cell lines, using Osteocalcin (BGLAP) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s. |
 | Western blot analysis of extracts of various cell lines, using Osteocalcin (BGLAP) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
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