Epigenetics & Nuclear Signaling Antibodies 4
Anti-OSGEPL1 Antibody (CAB8022)
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- SKU:
- CAB8022
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | OSGEPL1 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8022 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human OSGEPL1 (NP_071748.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse heart, Mouse kidney, Mouse liver, Mouse testis, Rat liver, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human OSGEPL1 (NP_071748.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLIL TKTA GVFF KPSK RKVY EFLR SFNF HPGT LFLH KIVL GIET SCDD TAAA VVDE TGNV LGEA IHSQ TEVH LKTG GIVP PAAQ QLHR ENIQ RIVQ EALS ASGV SPSD LSAI ATTI KPGL ALSL GVGL SFSL QLVG QLKK PFIP IHHM EAHA LTIR LTNK VEFP FLVL LISG GHCL LALV QGVS DFLL LGKS LDIA PGDM LDKV ARRL SLIK HPEC STMS GGKA IEHL AKQG NRFH FDIK PPLH HAKN CDFS FTGL QHVT DKII MKKE KE |
| Gene ID: | 64172 |
| Uniprot: | Q9H4B0 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 32kDa/39kDa/45kDa |
| Observed MW: | 45kDa |
| Synonyms: | OSGEPL1, OSGEPL, Qri7 |
| Background: |
| UniProt Protein Function: | OSGEPL1: Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Belongs to the KAE1 / YgjD family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.6.99.4; Protease Chromosomal Location of Human Ortholog: 2q32.2 Cellular Component: mitochondrion Molecular Function:metal ion binding; transferase activity, transferring groups other than amino-acyl groups |
| UniProt Code: | Q9H4B0 |
| NCBI GenInfo Identifier: | 116812636 |
| NCBI Gene ID: | 64172 |
| NCBI Accession: | NP_071748.2 |
| UniProt Secondary Accession: | Q9H4B0,Q96EV9, Q96NH5, |
| UniProt Related Accession: | Q9H4B0 |
| Molecular Weight: | 32,586 Da |
| NCBI Full Name: | probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial |
| NCBI Synonym Full Names: | O-sialoglycoprotein endopeptidase-like 1 |
| NCBI Official Symbol: | OSGEPL1 |
| NCBI Official Synonym Symbols: | Qri7 |
| NCBI Protein Information: | probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial |
| UniProt Protein Name: | Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial |
| UniProt Synonym Protein Names: | N6-L-threonylcarbamoyladenine synthase; t(6)A synthase; O-sialoglycoprotein endopeptidase-like protein 1 |
| UniProt Gene Name: | OSGEPL1 |
| UniProt Entry Name: | OSGP2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using OSGEPL1 antibody (CAB8022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human liver damage using OSGEPL1 antibody (CAB8022) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using OSGEPL1 antibody (CAB8022) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse stomach using OSGEPL1 antibody (CAB8022) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A549 cells using OSGEPL1 antibody (CAB8022). Blue: DAPI for nuclear staining. |
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