Name: Anti-OSBP Antibody (CAB3998)
Brand: Cell Biology Antibodies 9
SKU: CAB3998
Availability: OutOfStock

Antibody Name:	Anti-OSBP Antibody
Antibody SKU:	CAB3998
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 508-807 of human OSBP (NP_002547.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HeLa, BT-474, NIH/3T3, Mouse brain, Mouse kidney, Mouse liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 508-807 of human OSBP (NP_002547.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LEEN GYRS LCEQ VSHH PPAA AHHA ESKN GWTL RQEI KITS KFRG KYLS IMPL GTIH CIFH ATGH HYTW KKVT TTVH NIIV GKLW IDQS GEID IVNH KTGD KCNL KFVP YSYF SRDV ARKV TGEV TDPS GKVH FALL GTWD EKME CFKV QPVI GENG GDAR QRGH EAEE SRVM LWKR NPLP KNAE NMYY FSEL ALTL NAWE SGTA PTDS RLRP DQRL MENG RWDE ANAE KQRL EEKQ RLSR KKRE AEAM KATE DGTP YDPY KALW FERK KDPV TKEL THIY RGEY WECK EKQD WSSC PDIF
Gene ID:	5007
Uniprot:	P22059
Cellular Location:	Cytoplasm, Endoplasmic reticulum membrane, Golgi apparatus membrane, Peripheral membrane protein, cytosol, perinuclear region
Calculated MW:	89kDa
Observed MW:	110kDa

Synonyms:	OSBP, OSBP1
Background:	Oxysterol binding protein is an intracellular protein that is believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase

UniProt Protein Function:	OSBP: Binds cholesterol and a range of oxysterols. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylate ERK1/2, whereas 25- hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing ABCA1 stability. Belongs to the OSBP family.
UniProt Protein Details:	Protein type:Lipid-binding Chromosomal Location of Human Ortholog: 11q12-q13 Cellular Component: Golgi membrane; nucleoplasm; Golgi apparatus; cytoplasm; nucleolus; nucleus; cell junction Molecular Function:protein domain specific binding; protein binding; oxysterol binding Biological Process: lipid transport
NCBI Summary:	Oxysterol binding protein is an intracellular protein that is believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase [provided by RefSeq, Jul 2008]
UniProt Code:	P22059
NCBI GenInfo Identifier:	129308
NCBI Gene ID:	5007
NCBI Accession:	P22059.1
UniProt Secondary Accession:	P22059,Q6P524,
UniProt Related Accession:	P22059
Molecular Weight:	807
NCBI Full Name:	Oxysterol-binding protein 1
NCBI Synonym Full Names:	oxysterol binding protein
NCBI Official Symbol:	OSBP
NCBI Official Synonym Symbols:	OSBP1
NCBI Protein Information:	oxysterol-binding protein 1
UniProt Protein Name:	Oxysterol-binding protein 1
Protein Family:	Oxysterol-binding protein
UniProt Gene Name:	OSBP
UniProt Entry Name:	OSBP1_HUMAN

	Western blot analysis of extracts of various cell lines, using OSBP antibody (CAB3998) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of NIH-3T3 cells using OSBP Rabbit pAb (CAB3998) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.