Epigenetics & Nuclear Signaling Antibodies 4

Anti-ORC6 Antibody (CAB7966)

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SKU:
CAB7966
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:ORC6 Rabbit Polyclonal Antibody
Antibody SKU:CAB7966
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-252 of human ORC6 (NP_055136.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, SW620, PC-3, HeLa, Mouse liver, Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-252 of human ORC6 (NP_055136.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGSE LIGR LAPR LGLA EPDM LRKA EEYL RLSR VKCV GLSA RTTE TSSA VMCL DLAA SWMK CPLD RAYL IKLS GLNK ETYQ SCLK SFEC LLGL NSNI GIRD LAVQ FSCI EAVN MASK ILKS YESS LPQT QQVD LDLS RPLF TSAA LLSA CKIL KLKV DKNK MVAT SGVK KAIF DRLC KQLE KIGQ QVDR EPGD VATP PRKR KKIV VEAP AKEM EKVE EMPH KPQK DEDL TQDY EEWK RKIL ENAA SAQK ATAE
Gene ID:23594
Uniprot:Q9Y5N6
Cellular Location:Nucleus
Calculated MW:28kDa
Observed MW:28kDa
Synonyms:ORC6, ORC6L
Background:The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis.
UniProt Protein Function:ORC6L: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. ORC is composed of six subunits. In human, ORC is cell cycle-dependent regulated: it is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts with DBF4. Belongs to the ORC6 family.
UniProt Protein Details:

Protein type:DNA replication

Chromosomal Location of Human Ortholog: 16q12

Cellular Component: nucleoplasm; membrane; plasma membrane; nuclear origin of replication recognition complex; nucleus; origin recognition complex

Molecular Function:protein binding; DNA binding

Biological Process: mitotic cell cycle; DNA replication; DNA-dependent DNA replication; G1/S transition of mitotic cell cycle

Disease: Meier-gorlin Syndrome 3

NCBI Summary:The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis. [provided by RefSeq, Oct 2010]
UniProt Code:Q9Y5N6
NCBI GenInfo Identifier:8928274
NCBI Gene ID:23594
NCBI Accession:Q9Y5N6.1
UniProt Secondary Accession:Q9Y5N6,B3KN89,
UniProt Related Accession:Q9Y5N6
Molecular Weight:252
NCBI Full Name:Origin recognition complex subunit 6
NCBI Synonym Full Names:origin recognition complex, subunit 6
NCBI Official Symbol:ORC6  
NCBI Official Synonym Symbols:ORC6L  
NCBI Protein Information:origin recognition complex subunit 6
UniProt Protein Name:Origin recognition complex subunit 6
Protein Family:Origin recognition complex
UniProt Gene Name:ORC6  
UniProt Entry Name:ORC6_HUMAN
Anti-ORC6 Polyclonal Antibody (CAB7966)Western blot analysis of extracts of various cell lines, using ORC6 antibody (CAB7966) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 30s.
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