Epigenetics & Nuclear Signaling Antibodies 4

Anti-ORC4L Antibody (CAB7705)

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SKU:
CAB7705
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-ORC4L Antibody
Antibody SKU:CAB7705
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 187-436 of human ORC4L (NP_002543.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa, MCF7, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 187-436 of human ORC4L (NP_002543.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IAVI GLTC RLDI LELL EKRV KSRF SHRQ IHLM NSFG FPQY VKIF KEQL SLPA EFPD KVFA EKWN ENVQ YLSE DRSV QEVL QKHF NISK NLRS LHML LMLA LNRV TASH PFMT AVDL MEAS QLCS MDSK ANIV HGLS VLEI CLII AMKH LNDI YEEE PFNF QMVY NEFQ KFVQ RKAH SVYN FEKP VVMK AFEH LQQL ELIK PMER TSGN SQRE YQLM KLLL DNTQ IMNA LQKY PNCP TDVR QWAT SSLS WL
Gene ID:5000
Uniprot:O43929
Cellular Location:Nucleus
Calculated MW:40kDa/42kDa/50kDa
Observed MW:50kDa
Synonyms:ORC4, ORC4L, ORC4P
Background:The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. This gene encodes a subunit of the ORC complex. Several alternatively spliced transcript variants, some of which encode the same protein, have been reported for this gene.
UniProt Protein Function:Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3.
NCBI Summary:The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. This gene encodes a subunit of the ORC complex. Several alternatively spliced transcript variants, some of which encode the same protein, have been reported for this gene. [provided by RefSeq, Oct 2010]
UniProt Code:O43929
NCBI GenInfo Identifier:300116159
NCBI Gene ID:5000
NCBI Accession:NP_001177808.1
UniProt Secondary Accession:O43929,Q96C42, B7Z3D0, B7Z5F1, D3DP86, F5H069,
UniProt Related Accession:O43929
Molecular Weight:47
NCBI Full Name:origin recognition complex subunit 4 isoform 1
NCBI Synonym Full Names:origin recognition complex subunit 4
NCBI Official Symbol:ORC4  
NCBI Official Synonym Symbols:ORC4L; ORC4P  
NCBI Protein Information:origin recognition complex subunit 4
UniProt Protein Name:Origin recognition complex subunit 4
Protein Family:Origin recognition complex
UniProt Gene Name:ORC4  
Anti-ORC4L Antibody (CAB7705)Western blot analysis of extracts of various cell lines, using ORC4 antibody (CAB7705) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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