Epigenetics & Nuclear Signaling Antibodies 2

Anti-ORC3 Antibody (CAB15415)

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SKU:
CAB15415
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-ORC3 Antibody
Antibody SKU:CAB15415
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ORC3 (NP_036513.2).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:LO2, 293T, HeLa, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ORC3 (NP_036513.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MATS SMSK GCFV FKPN SKKR KISL PIED YFNK GKNE PEDS KLRF ETYQ LIWQ QMKS ENER LQEE LNKN LFDN LIEF LQKS HSGF QKNS RDLG GQIK LREI PTAA LVLG VNVT DHDL TFGS LTEA LQNN VTPY VVSL QAKD CPDM KHFL QKLI SQLM DCCV DIKS KEEE SVHV TQRK THYS MDSL SSWY MTVT QKTD PKML SKKR TTSS QWQS PPVV VILK DMES FATK VLQD FIII SSQH
Gene ID:23595
Uniprot:Q9UBD5
Cellular Location:Nucleus
Calculated MW:65kDa/82kDa
Observed MW:82kDa
Synonyms:ORC3, LAT, LATHEO, ORC3L
Background:The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Studies of a similar gene in Drosophila suggested a possible role of this protein in neuronal proliferation and olfactory memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene.
UniProt Protein Function:ORC3L: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Belongs to the ORC3 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell cycle regulation

Chromosomal Location of Human Ortholog: 6q14.3-q16.1

Cellular Component: nuclear chromosome, telomeric region; nuclear origin of replication recognition complex; nucleoplasm; origin recognition complex

Molecular Function:DNA replication origin binding; protein binding

Biological Process: DNA replication; G1/S transition of mitotic cell cycle

NCBI Summary:The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Studies of a similar gene in Drosophila suggested a possible role of this protein in neuronal proliferation and olfactory memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q9UBD5
NCBI GenInfo Identifier:8928268
NCBI Gene ID:23595
NCBI Accession:Q9UBD5.1
UniProt Secondary Accession:Q9UBD5,Q13565, Q53GY6, Q5T159, Q6IUY7, Q86TN5, Q9UG44 Q9UNT6, A2A2T5, B4E025,
UniProt Related Accession:Q9UBD5
Molecular Weight:65,929 Da
NCBI Full Name:Origin recognition complex subunit 3
NCBI Synonym Full Names:origin recognition complex subunit 3
NCBI Official Symbol:ORC3  
NCBI Official Synonym Symbols:LAT; ORC3L; LATHEO  
NCBI Protein Information:origin recognition complex subunit 3
UniProt Protein Name:Origin recognition complex subunit 3
UniProt Synonym Protein Names:Origin recognition complex subunit Latheo
Protein Family:Origin recognition complex
UniProt Gene Name:ORC3  
UniProt Entry Name:ORC3_HUMAN
Anti-ORC3 Antibody (CAB15415)Western blot analysis of extracts of various cell lines, using ORC3 antibody (CAB15415) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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