Name: Anti-ORC2 Antibody (CAB15697)
Brand: Epigenetics & Nuclear Signaling Antibodies 2
SKU: CAB15697
Availability: OutOfStock

Antibody Name:	Anti-ORC2 Antibody
Antibody SKU:	CAB15697
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 458-577 of human ORC2 (NP_006181.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	HeLa, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 458-577 of human ORC2 (NP_006181.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TSYE NSLL VKQS GSLP LSSL THVL RSLT PNAR GIFR LLIK YQLD NQDN PSYI GLSF QDFY QQCR EAFL VNSD LTLR AQLT EFRD HKLI RTKK GTDG VEYL LIPV DNGT LTDF LEKE EEEA
Gene ID:	4999
Uniprot:	Q13416
Cellular Location:	Nucleus
Calculated MW:	65kDa
Observed MW:	80kDa

Synonyms:

ORC2, ORC2L

Background:

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3, -4, and -5. It also interacts with CDC45 and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication. Alternatively spliced transcript variants have been found, one of which is a nonsense-mediated mRNA decay candidate.

UniProt Protein Function:	ORC2: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. ORC is composed of six subunits. In human, ORC is cell cycle-dependent regulated: it is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts with DBF4. Interacts with MCM10. Belongs to the ORC2 family.
UniProt Protein Details:	Protein type:DNA replication Chromosomal Location of Human Ortholog: 2q33 Cellular Component: centrosome; heterochromatin; membrane; nuclear chromosome, telomeric region; nuclear origin of replication recognition complex; nucleoplasm; nucleus; origin recognition complex Molecular Function:protein binding Biological Process: DNA replication; DNA replication initiation; G1/S transition of mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter
NCBI Summary:	The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3, -4, and -5. It also interacts with CDC45 and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication. Alternatively spliced transcript variants have been found, one of which is a nonsense-mediated mRNA decay candidate. [provided by RefSeq, Oct 2010]
UniProt Code:	Q13416
NCBI GenInfo Identifier:	8488999
NCBI Gene ID:	4999
NCBI Accession:	Q13416.2
UniProt Secondary Accession:	Q13416,Q13204, Q53TX5,
UniProt Related Accession:	Q13416
Molecular Weight:	65,972 Da
NCBI Full Name:	Origin recognition complex subunit 2
NCBI Synonym Full Names:	origin recognition complex subunit 2
NCBI Official Symbol:	ORC2
NCBI Official Synonym Symbols:	ORC2L
NCBI Protein Information:	origin recognition complex subunit 2
UniProt Protein Name:	Origin recognition complex subunit 2
Protein Family:	Origin recognition complex
UniProt Gene Name:	ORC2
UniProt Entry Name:	ORC2_HUMAN

Western blot analysis of extracts of various cell lines, using ORC2 antibody (CAB15697) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

ORC2 Antibody (PACO11012)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)