Name: Anti-ORAI1 Antibody (CAB7412)
Brand: Immunology Antibodies 2
SKU: CAB7412
Availability: OutOfStock

Antibody Name:	Anti-ORAI1 Antibody
Antibody SKU:	CAB7412
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 202-301 of human ORAI1 (NP_116179.2).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	SKOV3, DU145, HeLa, MCF7, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 202-301 of human ORAI1 (NP_116179.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LKKQ PGQP RPTS KPPA SGAA ANVS TSGI TPGQ AAAI ASTT IMVP FGLI FIVF AVHF YRSL VSHK TDRQ FQEL NELA EFAR LQDQ LDHR GDHP LTPG SHYA
Gene ID:	84876
Uniprot:	Q96D31
Cellular Location:	Cell membrane, Cytoplasmic vesicle, Multi-pass membrane protein, autophagosome
Calculated MW:	25kDa/32kDa
Observed MW:	37kDa

Synonyms:	CRACM1, IMD9, ORAT1, TAM2, TMEM142A, ORAI1
Background:	The protein encoded by this gene is a membrane calcium channel subunit that is activated by the calcium sensor STIM1 when calcium stores are depleted. This type of channel is the primary way for calcium influx into T-cells. Defects in this gene are a cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1).

UniProt Protein Function:	ORAI1: Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. Interacts with STIM1 and STIM2. Interacts with EFCAB4B/CRACR2A; the interaction is direct and takes place in absence of Ca(2+). Forms a complex with EFCAB4B/CRACR2A and STIM1 at low concentration of Ca(2+), the complex dissociates at elevated Ca(2+) concentrations. Belongs to the Orai family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Membrane protein, multi-pass; Channel, calcium Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: protein complex; membrane; integral to plasma membrane Molecular Function:calmodulin binding; identical protein binding; protein binding; store-operated calcium channel activity Biological Process: immune system process; regulation of calcium ion transport; positive regulation of calcium ion transport; blood coagulation Disease: Myopathy, Tubular Aggregate, 2; Immunodeficiency 9
NCBI Summary:	The protein encoded by this gene is a membrane calcium channel subunit that is activated by the calcium sensor STIM1 when calcium stores are depleted. This type of channel is the primary way for calcium influx into T-cells. Defects in this gene are a cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1). [provided by RefSeq, Sep 2011]
UniProt Code:	Q96D31
NCBI GenInfo Identifier:	97180269
NCBI Gene ID:	84876
NCBI Accession:	Q96D31.2
UniProt Secondary Accession:	Q96D31,Q5M848, Q8BWG9, Q6TLE6, Q5ZL05,
UniProt Related Accession:	Q96D31
Molecular Weight:	25,337 Da
NCBI Full Name:	Calcium release-activated calcium channel protein 1
NCBI Synonym Full Names:	ORAI calcium release-activated calcium modulator 1
NCBI Official Symbol:	ORAI1
NCBI Official Synonym Symbols:	IMD9; TAM2; ORAT1; CRACM1; TMEM142A
NCBI Protein Information:	calcium release-activated calcium channel protein 1; protein orai-1; transmembrane protein 142A; calcium release-activated calcium modulator 1
UniProt Protein Name:	Calcium release-activated calcium channel protein 1
UniProt Synonym Protein Names:	Protein orai-1; Transmembrane protein 142A
UniProt Gene Name:	ORAI1
UniProt Entry Name:	CRCM1_HUMAN

Western blot analysis of extracts of various cell lines, using ORAI1 antibody (CAB7412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

ORAI1 Antibody (PACO11011)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)