Cell Biology Antibodies 7

Anti-OGFOD1 Antibody (CAB16543)

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SKU:
CAB16543
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-OGFOD1 Antibody
Antibody SKU:CAB16543
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human OGFOD1 (NP_060703.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human OGFOD1 (NP_060703.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MNGK RPAE PGPA RVGK KGKK EVMA EFSD AVTE ETLK KQVA EAWS RRTP FSHE VIVM DMDP FLHC VIPN FIQS QDFL EGLQ KELM NLDF HEKY NDLY KFQQ SDDL KKRR EPHI STLR KILF EDFR SWLS DISK IDLE STID
Gene ID:55239
Uniprot:Q8N543
Cellular Location:Cytoplasm, Nucleus
Calculated MW:46kDa/63kDa
Observed MW:63kDa
Synonyms:OGFOD1, TPA1
Background:
UniProt Protein Function:OGFOD1: Belongs to the TPA1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 1.14.11.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: stress granule; cytoplasm; nucleus

Molecular Function:peptidyl-proline dioxygenase activity; L-ascorbic acid binding; iron ion binding; peptidyl-proline 3-dioxygenase activity

Biological Process: cell proliferation; stress granule assembly; peptidyl-proline hydroxylation; protein amino acid hydroxylation; regulation of translational termination

UniProt Code:Q8N543
NCBI GenInfo Identifier:94536836
NCBI Gene ID:55239
NCBI Accession:NP_060703.3
UniProt Secondary Accession:Q8N543,Q9H7U5, Q9H9J9, Q9HA87, Q9HCG0, Q9NVB6, H3BUQ2
UniProt Related Accession:Q8N543
Molecular Weight:46,861 Da
NCBI Full Name:prolyl 3-hydroxylase OGFOD1
NCBI Synonym Full Names:2-oxoglutarate and iron-dependent oxygenase domain containing 1
NCBI Official Symbol:OGFOD1  
NCBI Official Synonym Symbols:TPA1  
NCBI Protein Information:prolyl 3-hydroxylase OGFOD1; termination and polyadenylation 1 homolog; TPA1, termination and polyadenylation 1, homolog; 2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1
UniProt Protein Name:Prolyl 3-hydroxylase OGFOD1
UniProt Synonym Protein Names:2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1; Termination and polyadenylation 1 homolog
Protein Family:Prolyl 3-hydroxylase
UniProt Gene Name:OGFOD1  
UniProt Entry Name:OGFD1_HUMAN
Anti-OGFOD1 Antibody (CAB16543)Western blot analysis of extracts of HeLa cells, using OGFOD1 antibody (CAB16543) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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