Metabolism Antibodies 1
Anti-OGDH Antibody (CAB12477)
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- SKU:
- CAB12477
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-OGDH Antibody |
| Antibody SKU: | CAB12477 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 148-427 of human OGDH (NP_001003941.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse kidney, Mouse heart, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 148-427 of human OGDH (NP_001003941.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DPLG ILDA DLDS SVPA DIIS STDK LGFY GLDE SDLD KVFH LPTT TFIG GQES ALPL REII RRLE MAYC QHIG VEFM FIND LEQC QWIR QKFE TPGI MQFT NEEK RTLL ARLV RSTR FEEF LQRK WSSE KRFG LEGC EVLI PALK TIID KSSE NGVD YVIM GMPH RGRL NVLA NVIR KELE QIFC QFDS KLEA ADEG SGDV KYHL GMYH RRIN RVTD RNIT LSLV ANPS HLEA ADPV VMGK TKAE QFYC GDTE GKKV RPRE RRAR QIVK APCS SMEF RSPT |
| Gene ID: | 4967 |
| Uniprot: | Q02218 |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 48kDa/115kDa |
| Observed MW: | 116kDa |
| Synonyms: | OGDH, AKGDH, E1k, OGDC |
| Background: | This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| UniProt Protein Function: | OGDH: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). Belongs to the alpha-ketoglutarate dehydrogenase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - lysine degradation; Oxidoreductase; Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial; EC 1.2.4.2 Chromosomal Location of Human Ortholog: 7p14-p13 Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial membrane; cytosol; oxoglutarate dehydrogenase complex Molecular Function:chaperone binding; metal ion binding; heat shock protein binding; thiamin pyrophosphate binding; oxoglutarate dehydrogenase (succinyl-transferring) activity Biological Process: generation of precursor metabolites and energy; glycolysis; striatum development; succinyl-CoA metabolic process; tricarboxylic acid cycle; hippocampus development; pyramidal neuron development; thalamus development; 2-oxoglutarate metabolic process; cellular metabolic process; cerebellar cortex development; tangential migration from the subventricular zone to the olfactory bulb; NADH metabolic process; lysine catabolic process Disease: Alpha-ketoglutarate Dehydrogenase Deficiency |
| NCBI Summary: | This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009] |
| UniProt Code: | Q02218 |
| NCBI GenInfo Identifier: | 160332299 |
| NCBI Gene ID: | 4967 |
| NCBI Accession: | Q02218.3 |
| UniProt Secondary Accession: | Q02218,Q96DD3, Q9UDX0, B4E2U9, D3DVL0, E9PBM1, |
| UniProt Related Accession: | Q02218 |
| Molecular Weight: | 1023 |
| NCBI Full Name: | 2-oxoglutarate dehydrogenase, mitochondrial |
| NCBI Synonym Full Names: | oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) |
| NCBI Official Symbol: | OGDH |
| NCBI Official Synonym Symbols: | E1k; OGDC; AKGDH |
| NCBI Protein Information: | 2-oxoglutarate dehydrogenase, mitochondrial; OGDC-E1; oxoglutarate decarboxylase; 2-oxoglutarate dehydrogenase complex component E1; oxoglutarate dehydrogenase (succinyl-transferring) |
| UniProt Protein Name: | 2-oxoglutarate dehydrogenase, mitochondrial |
| UniProt Synonym Protein Names: | 2-oxoglutarate dehydrogenase complex component E1; OGDC-E1; Alpha-ketoglutarate dehydrogenase |
| UniProt Gene Name: | OGDH |
| UniProt Entry Name: | ODO1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using OGDH Antibody (CAB12477) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using OGDH antibody (CAB12477) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using OGDH antibody (CAB12477) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using OGDH antibody (CAB12477). Blue: DAPI for nuclear staining. |
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