Cell Biology Antibodies 12

Anti-OCRL Antibody (CAB9290)

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SKU:
CAB9290
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-OCRL Antibody
Antibody SKU:CAB9290
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human OCRL (NP_001578.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-251MG, A-549, 22Rv1, Mouse brain, Mouse lung, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human OCRL (NP_001578.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEPP LPVG AQPL ATVE GMEM KGPL REPC ALTL AQRN GQYE LIIQ LHEK EQHV QDII PINS HFRC VQEA EETL LIDI ASNS GCKI RVQG DWIR ERRF EIPD EEHC LKFL SAVL AAQK AQSQ LLVP EQKD SSSW YQKL DTKD KPSV FSGL LGFE DNFS SMNL DKKI NSQN QPTG IHRE PPPP PFSV NKML PREK EASN KEQP KVTN TMRK LFVP NTQS GQRE
Gene ID:4952
Uniprot:Q01968
Cellular Location:Cell projection, Cytoplasmic vesicle, Early endosome membrane, Endosome, Golgi apparatus, Membrane, cilium, clathrin-coated pit, phagosome membrane, photoreceptor outer segment, trans-Golgi network
Calculated MW:103kDa/104kDa
Observed MW:110kDa
Synonyms:OCRL, INPP5F, LOCR, NPHL2, OCRL-1, OCRL1
Background:This gene encodes an inositol polyphosphate 5-phosphatase. This protein is involved in regulating membrane trafficking and is located in numerous subcellular locations including the trans-Golgi network, clathrin-coated vesicles and, endosomes and the plasma membrane. This protein may also play a role in primary cilium formation. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:Converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate. Also converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate (PubMed:25869668, PubMed:7761412, PubMed:9430698). May function in lysosomal membrane trafficking by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with lysosomes. Involved in primary cilia assembly (PubMed:22228094, PubMed:22543976).
NCBI Summary:This gene encodes an inositol polyphosphate 5-phosphatase. This protein is involved in regulating membrane trafficking and is located in numerous subcellular locations including the trans-Golgi network, clathrin-coated vesicles and, endosomes and the plasma membrane. This protein may also play a role in primary cilium formation. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
UniProt Code:Q01968
NCBI GenInfo Identifier:13325072
NCBI Gene ID:4952
NCBI Accession:NP_000267.2
UniProt Related Accession:Q01968
Molecular Weight:
NCBI Full Name:inositol polyphosphate 5-phosphatase OCRL isoform a
NCBI Synonym Full Names:OCRL inositol polyphosphate-5-phosphatase
NCBI Official Symbol:OCRL  
NCBI Official Synonym Symbols:LOCR; NPHL2; OCRL1; Dent-2; INPP5F; OCRL-1  
NCBI Protein Information:inositol polyphosphate 5-phosphatase OCRL
UniProt Protein Name:Inositol polyphosphate 5-phosphatase OCRL-1
UniProt Synonym Protein Names:Lowe oculocerebrorenal syndrome protein
Protein Family:Inositol polyphosphate 5-phosphatase
UniProt Gene Name:OCRL  
Anti-OCRL Polyclonal Antibody (CAB9290)Western blot analysis of extracts of various cell lines, using OCRL antibody (CAB9290) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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