KO Validated Antibodies 2

Anti-OCRL Antibody (CAB19976)[KO Validated]

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SKU:
CAB19976
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-OCRL Antibody [KO Validated]
Antibody SKU:CAB19976
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human OCRL.
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant protein of human OCRL.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:4952
Uniprot:Q01968
Cellular Location:Cell projection, Cytoplasmic vesicle, Early endosome membrane, Endosome, Golgi apparatus, Membrane, cilium, clathrin-coated pit, phagosome membrane, photoreceptor outer segment, trans-Golgi network
Calculated MW:103kDa/104kDa
Observed MW:104KDa
Anti-OCRL Antibody [KO Validated] (CAB19976)Western blot analysis of extracts from normal (control) and OCRL knockout (KO) 293T cells, using OCRL antibody (CAB19976) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Synonyms:OCRL, INPP5F, LOCR, NPHL2, OCRL-1, OCRL1
Background:This gene encodes an inositol polyphosphate 5-phosphatase. This protein is involved in regulating membrane trafficking and is located in numerous subcellular locations including the trans-Golgi network, clathrin-coated vesicles and, endosomes and the plasma membrane. This protein may also play a role in primary cilium formation. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:Converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate. Also converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate (PubMed:25869668, PubMed:7761412, PubMed:9430698). May function in lysosomal membrane trafficking by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with lysosomes. Involved in primary cilia assembly (PubMed:22228094, PubMed:22543976).
NCBI Summary:This gene encodes an inositol polyphosphate 5-phosphatase. This protein is involved in regulating membrane trafficking and is located in numerous subcellular locations including the trans-Golgi network, clathrin-coated vesicles and, endosomes and the plasma membrane. This protein may also play a role in primary cilium formation. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
UniProt Code:Q01968
NCBI GenInfo Identifier:13325072
NCBI Gene ID:4952
NCBI Accession:NP_000267.2
UniProt Related Accession:Q01968
Molecular Weight:
NCBI Full Name:inositol polyphosphate 5-phosphatase OCRL isoform a
NCBI Synonym Full Names:OCRL inositol polyphosphate-5-phosphatase
NCBI Official Symbol:OCRL  
NCBI Official Synonym Symbols:LOCR; NPHL2; OCRL1; Dent-2; INPP5F; OCRL-1  
NCBI Protein Information:inositol polyphosphate 5-phosphatase OCRL
UniProt Protein Name:Inositol polyphosphate 5-phosphatase OCRL-1
UniProt Synonym Protein Names:Lowe oculocerebrorenal syndrome protein
Protein Family:Inositol polyphosphate 5-phosphatase
UniProt Gene Name:OCRL  
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