Cell Biology Antibodies 3
Anti-Occludin Antibody (CAB12621)
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- SKU:
- CAB12621
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Occludin Antibody |
| Antibody SKU: | CAB12621 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 350-450 of human Occludin (NP_002529.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | DU145, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 350-450 of human Occludin (NP_002529.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VVQE LPLT SPVD DFRQ PRYS SGGN FETP SKRA PAKG RAGR SKRT EQDH YETD YTTG GESC DELE EDWI REYP PITS DQQR QLYK RNFD TGLQ EYKS LQSE L |
| Gene ID: | 100506658 |
| Uniprot: | Q16625 |
| Cellular Location: | Cell junction, Membrane, Multi-pass membrane protein, tight junction |
| Calculated MW: | 8kDa/23kDa/31kDa/52kDa/54kDa/59kDa |
| Observed MW: | 68kDa |
| Synonyms: | OCLN, BLCPMG, PPP1R115, PTORCH1, occludin, Occludin |
| Background: | This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. |
| UniProt Protein Function: | occludin: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. Interacts with TJP1/ZO1 and with VAPA. Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Belongs to the ELL/occludin family. 7 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 5q13.1 Cellular Component: tight junction; endocytic vesicle; apicolateral plasma membrane; apical plasma membrane; integral to membrane; plasma membrane; cytoplasmic vesicle; intercellular junction; cytosol; cell junction Molecular Function:thiopurine S-methyltransferase activity; protein domain specific binding; protein binding; structural molecule activity Biological Process: methylation; intercellular junction assembly and maintenance; apoptosis; S-adenosylmethionine metabolic process; protein complex assembly; S-adenosylhomocysteine metabolic process; cell structure disassembly during apoptosis Disease: Band-like Calcification With Simplified Gyration And Polymicrogyria |
| NCBI Summary: | This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011] |
| UniProt Code: | Q16625 |
| NCBI GenInfo Identifier: | 3914196 |
| NCBI Gene ID: | 100506658 |
| NCBI Accession: | Q16625.1 |
| UniProt Secondary Accession: | Q16625,Q5U1V4, Q8N6K1, B5BU70, D2DU64, D2DU65, D2IGC0 D2IGC1, E2CYV9, |
| UniProt Related Accession: | Q16625 |
| Molecular Weight: | 522 |
| NCBI Full Name: | Occludin |
| NCBI Synonym Full Names: | occludin |
| NCBI Official Symbol: | OCLN |
| NCBI Official Synonym Symbols: | BLCPMG; PPP1R115 |
| NCBI Protein Information: | occludin; tight junction protein occludin; phosphatase 1, regulatory subunit 115 |
| UniProt Protein Name: | Occludin |
| Protein Family: | Occludin |
| UniProt Gene Name: | OCLN |
| UniProt Entry Name: | OCLN_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Occludin antibody (CAB12621) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunohistochemistry of paraffin-embedded human stomach using Occludin antibody (CAB12621) at dilution of 1:100 (40x lens). |
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