Cell Biology Antibodies 10
Anti-OAT Antibody (CAB6235)
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- SKU:
- CAB6235
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-OAT Antibody |
| Antibody SKU: | CAB6235 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 160-439 of human OAT (NP_000265.1). |
| Application: | WB IHC IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW480, MCF7, 293T, A-549, HepG2, Mouse liver, Mouse lung, Mouse kidney, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 160-439 of human OAT (NP_000265.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VKGI QKYK AKIV FAAG NFWG RTLS AISS STDP TSYD GFGP FMPG FDII PYND LPAL ERAL QDPN VAAF MVEP IQGE AGVV VPDP GYLM GVRE LCTR HQVL FIAD EIQT GLAR TGRW LAVD YENV RPDI VLLG KALS GGLY PVSA VLCD DDIM LTIK PGEH GSTY GGNP LGCR VAIA ALEV LEEE NLAE NADK LGII LRNE LMKL PSDV VTAV RGKG LLNA IVIK ETKD WDAW KVCL RLRD NGLL AKPT HGDI IRFA PPLV IKED ELRE SIEI INKT ILSF |
| Gene ID: | 4942 |
| Uniprot: | P04181 |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 32kDa/48kDa |
| Observed MW: | 47kDa |
| Synonyms: | OAT, GACR, HOGA, OATASE, OKT |
| Background: | This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome. |
| UniProt Protein Function: | OAT: Defects in OAT are the cause of hyperornithinemia with gyrate atrophy of choroid and retina (HOGA). HOGA is a slowly progressive blinding autosomal recessive disorder. Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - arginine and proline; EC 2.6.1.13; Transferase; Mitochondrial Chromosomal Location of Human Ortholog: 10q26 Cellular Component: mitochondrial matrix; mitochondrion Molecular Function:identical protein binding; ornithine-oxo-acid transaminase activity; pyridoxal phosphate binding Biological Process: amino acid biosynthetic process; arginine catabolic process to glutamate; arginine catabolic process to proline via ornithine; visual perception Disease: Gyrate Atrophy Of Choroid And Retina |
| NCBI Summary: | This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome. [provided by RefSeq, Jan 2010] |
| UniProt Code: | P04181 |
| NCBI GenInfo Identifier: | 129018 |
| NCBI Gene ID: | 4942 |
| NCBI Accession: | P04181.1 |
| UniProt Secondary Accession: | P04181,Q16068, Q16069, Q68CS0, Q6IAV9, Q9UD03, D3DRF0 |
| UniProt Related Accession: | P04181 |
| Molecular Weight: | 32,853 Da |
| NCBI Full Name: | Ornithine aminotransferase, mitochondrial |
| NCBI Synonym Full Names: | ornithine aminotransferase |
| NCBI Official Symbol: | OAT |
| NCBI Official Synonym Symbols: | OKT; GACR; HOGA; OATASE |
| NCBI Protein Information: | ornithine aminotransferase, mitochondrial |
| UniProt Protein Name: | Ornithine aminotransferase, mitochondrial |
| UniProt Synonym Protein Names: | Ornithine delta-aminotransferase; Ornithine--oxo-acid aminotransferase |
| UniProt Gene Name: | OAT |
| UniProt Entry Name: | OAT_HUMAN |
 | Western blot analysis of extracts of various cell lines, using OAT antibody (CAB6235) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using OAT Rabbit pAb (CAB6235) at dilution of 1:150 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human esophageal cancer using OAT Rabbit pAb (CAB6235) at dilution of 1:150 (40x lens). |
 | Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug OAT antibody (CAB6235). Western blot was performed from the immunoprecipitate using OAT antibody (CAB6235) at a dilition of 1:1000. |
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