Immunology Antibodies 2

Anti-OAS2 Antibody (CAB17428)

(No reviews yet) Write a Review
SKU:
CAB17428
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-OAS2 Antibody
Antibody SKU:CAB17428
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant protein of human OAS2.
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:Raji
Immunogen:Recombinant protein of human OAS2.
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:4939
Uniprot:P29728
Cellular Location:Cytoplasm, Endoplasmic reticulum, Microsome, Mitochondrion, Nucleus, perinuclear region
Calculated MW:19kDa/78kDa/82kDa
Observed MW:72kDa
Synonyms:OAS2
Background:This gene encodes a member of the 2-5A synthetase family, essential proteins involved in the innate immune response to viral infection. The encoded protein is induced by interferons and uses adenosine triphosphate in 2'-specific nucleotidyl transfer reactions to synthesize 2', 5'-oligoadenylates (2-5As). These molecules activate latent RNase L, which results in viral RNA degradation and the inhibition of viral replication. The three known members of this gene family are located in a cluster on chromosome 12. Alternatively spliced transcript variants encoding different isoforms have been described.
UniProt Protein Function:OAS2: Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. Belongs to the 2-5A synthase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 2.7.7.84; Transferase

Chromosomal Location of Human Ortholog: 12q24.2

Cellular Component: mitochondrion; intracellular membrane-bound organelle; membrane; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; nucleus; cytosol

Molecular Function:zinc ion binding; metal ion binding; double-stranded RNA binding; ATP binding; 2'-5'-oligoadenylate synthetase activity

Biological Process: cytokine and chemokine mediated signaling pathway; response to virus; RNA catabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; protein myristoylation; protein amino acid glycosylation; purine nucleotide biosynthetic process; defense response to virus

NCBI Summary:This gene encodes a member of the 2-5A synthetase family, essential proteins involved in the innate immune response to viral infection. The encoded protein is induced by interferons and uses adenosine triphosphate in 2'-specific nucleotidyl transfer reactions to synthesize 2',5'-oligoadenylates (2-5As). These molecules activate latent RNase L, which results in viral RNA degradation and the inhibition of viral replication. The three known members of this gene family are located in a cluster on chromosome 12. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:P29728
NCBI GenInfo Identifier:116242687
NCBI Gene ID:4939
NCBI Accession:P29728.3
UniProt Secondary Accession:P29728,Q6PJ33, Q86XX8, A8K9T1,
UniProt Related Accession:P29728
Molecular Weight:719
NCBI Full Name:2'-5'-oligoadenylate synthase 2
NCBI Synonym Full Names:2'-5'-oligoadenylate synthetase 2, 69/71kDa
NCBI Official Symbol:OAS2  
NCBI Protein Information:2'-5'-oligoadenylate synthase 2; 2-5A synthase 2; p69 OAS / p71 OAS; (2'-5')oligo(A) synthetase 2
UniProt Protein Name:2'-5'-oligoadenylate synthase 2
UniProt Synonym Protein Names:p69 OAS / p71 OAS; p69OAS / p71OAS
Protein Family:2'-5'-oligoadenylate synthase
UniProt Gene Name:OAS2  
UniProt Entry Name:OAS2_HUMAN
Anti-OAS2 Antibody (CAB17428)Western blot analysis of extracts of Raji cells, using OAS2 antibody (CAB17428) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
View AllClose

0 Reviews

View AllClose

Related Products