Antibody Name:	Anti-OAS1 Antibody
Antibody SKU:	CAB2530
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-364 of human OAS1 (NP_002525.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-364 of human OAS1 (NP_002525.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MMDL RNTP AKSL DKFI EDYL LPDT CFRM QINH AIDI ICGF LKER CFRG SSYP VCVS KVVK GGSS GKGT TLRG RSDA DLVV FLSP LTTF QDQL NRRG EFIQ EIRR QLEA CQRE RAFS VKFE VQAP RWGN PRAL SFVL SSLQ LGEG VEFD VLPA FDAL GQLT GGYK PNPQ IYVK LIEE CTDL QKEG EFST CFTE LQRD FLKQ RPTK LKSL IRLV KHWY QNCK KKLG KLPP QYAL ELLT VYAW ERGS MKTH FNTA QGFR TVLE LVIN YQQL CIYW TKYY DFKN PIIE KYLR RQLT KPRP VILD PADP TGNL GGGD PKGW RQLA QEAE AWLN YPCF KNWD GSPV SSWI LLVR PPAS SLPF IPAP LHEA
Gene ID:	4938
Uniprot:	P00973
Cellular Location:	Cytoplasm, Endoplasmic reticulum, Microsome, Mitochondrion, Nucleus, Secreted
Calculated MW:	41kDa/43kDa/46kDa/47kDa
Observed MW:	40kDa

Synonyms:

OAS1, E18/E16, IFI-4, OIAS, OIASI

Background:

This gene is induced by interferons and encodes a protein that synthesizes 2', 5'-oligoadenylates (2-5As). This protein activates latent RNase L, which results in viral RNA degradation and the inhibition of viral replication. Alternative splicing results in multiple transcript variants with different enzymatic activities. Polymorphisms in this gene have been associated with susceptibility to viral infection and diabetes mellitus, type 1. A disease-associated allele in a splice acceptor site influences the production of the p46 splice isoform. This gene is located in a cluster of related genes on chromosome 12.

UniProt Protein Function:	OAS1: Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. The secreted form displays antiviral effect against vesicular stomatitis virus (VSV), herpes simplex virus type 2 (HSV-2), and encephalomyocarditis virus (EMCV) and stimulates the alternative antiviral pathway independent of RNase L. Belongs to the 2-5A synthase family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transferase; EC 2.7.7.84 Chromosomal Location of Human Ortholog: 12q24.2 Cellular Component: mitochondrion; endoplasmic reticulum; cytoplasm; extracellular region; cytosol; nucleus Molecular Function:protein binding; zinc ion binding; metal ion binding; double-stranded RNA binding; ATP binding; 2'-5'-oligoadenylate synthetase activity Biological Process: negative regulation of viral genome replication; response to virus; cytokine and chemokine mediated signaling pathway; glucose metabolic process; purine nucleotide biosynthetic process; glucose homeostasis; defense response to virus; protein oligomerization Disease: Diabetes Mellitus, Insulin-dependent
NCBI Summary:	This gene encodes a member of the 2-5A synthetase family, essential proteins involved in the innate immune response to viral infection. The encoded protein is induced by interferons and uses adenosine triphosphate in 2'-specific nucleotidyl transfer reactions to synthesize 2',5'-oligoadenylates (2-5As). These molecules activate latent RNase L, which results in viral RNA degradation and the inhibition of viral replication. The three known members of this gene family are located in a cluster on chromosome 12. Mutations in this gene have been associated with host susceptibility to viral infection. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	P00973
NCBI GenInfo Identifier:	296439492
NCBI Gene ID:	4938
NCBI Accession:	P00973.4
UniProt Secondary Accession:	P00973,P04820, P29080, P29081, P78485, P78486, Q16700 Q16701, Q1PG42, Q3ZM01, Q53GC5, A8K4N8,
UniProt Related Accession:	P00973
Molecular Weight:	400
NCBI Full Name:	2'-5'-oligoadenylate synthase 1
NCBI Synonym Full Names:	2'-5'-oligoadenylate synthetase 1, 40/46kDa
NCBI Official Symbol:	OAS1
NCBI Official Synonym Symbols:	OIAS; IFI-4; OIASI
NCBI Protein Information:	2'-5'-oligoadenylate synthase 1; E18/E16; p46/p42 OAS; 2-5A synthase 1; 2-5A synthetase 1; (2-5')oligo(A) synthase 1; 2',5'-oligo A synthetase 1; (2-5')oligo(A) synthetase 1; 2'-5'-oligoisoadenylate synthetase 1; 2',5'-oligoadenylate synthetase 1, 40/46kDa; 2'-5' oligoadenylate synthetase 1 p48 isoform; 2'-5' oligoadenylate synthetase 1 p52 isoform
UniProt Protein Name:	2'-5'-oligoadenylate synthase 1
UniProt Synonym Protein Names:	E18/E16; p46/p42 OAS
Protein Family:	2'-5'-oligoadenylate synthase
UniProt Gene Name:	OAS1
UniProt Entry Name:	OAS1_HUMAN

	Western blot analysis of extracts of mouse liver, using OAS1 antibody (CAB2530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of HeLa cells using OAS1 antibody (CAB2530).