Immunology Antibodies 1

Anti-OAS1 Antibody (CAB14623)

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SKU:
CAB14623
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-OAS1 Antibody
Antibody SKU:CAB14623
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human OAS1 (NP_002525.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse brain, Rat brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human OAS1 (NP_002525.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MMDL RNTP AKSL DKFI EDYL LPDT CFRM QINH AIDI ICGF LKER CFRG SSYP VCVS KVVK GGSS GKGT TLRG RSDA DLVV FLSP LTTF QDQL NRRG EFIQ
Gene ID:4938
Uniprot:P00973
Cellular Location:Cytoplasm, Endoplasmic reticulum, Microsome, Mitochondrion, Nucleus, Secreted
Calculated MW:41kDa/43kDa/46kDa/47kDa
Observed MW:46kDa
Synonyms:OAS1, E18/E16, IFI-4, OIAS, OIASI
Background:This gene is induced by interferons and encodes a protein that synthesizes 2', 5'-oligoadenylates (2-5As). This protein activates latent RNase L, which results in viral RNA degradation and the inhibition of viral replication. Alternative splicing results in multiple transcript variants with different enzymatic activities. Polymorphisms in this gene have been associated with susceptibility to viral infection and diabetes mellitus, type 1. A disease-associated allele in a splice acceptor site influences the production of the p46 splice isoform. This gene is located in a cluster of related genes on chromosome 12.
UniProt Protein Function:OAS1: Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. The secreted form displays antiviral effect against vesicular stomatitis virus (VSV), herpes simplex virus type 2 (HSV-2), and encephalomyocarditis virus (EMCV) and stimulates the alternative antiviral pathway independent of RNase L. Belongs to the 2-5A synthase family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transferase; EC 2.7.7.84

Chromosomal Location of Human Ortholog: 12q24.2

Cellular Component: mitochondrion; endoplasmic reticulum; cytoplasm; extracellular region; cytosol; nucleus

Molecular Function:protein binding; zinc ion binding; metal ion binding; double-stranded RNA binding; ATP binding; 2'-5'-oligoadenylate synthetase activity

Biological Process: negative regulation of viral genome replication; response to virus; cytokine and chemokine mediated signaling pathway; glucose metabolic process; purine nucleotide biosynthetic process; glucose homeostasis; defense response to virus; protein oligomerization

Disease: Diabetes Mellitus, Insulin-dependent

NCBI Summary:This gene encodes a member of the 2-5A synthetase family, essential proteins involved in the innate immune response to viral infection. The encoded protein is induced by interferons and uses adenosine triphosphate in 2'-specific nucleotidyl transfer reactions to synthesize 2',5'-oligoadenylates (2-5As). These molecules activate latent RNase L, which results in viral RNA degradation and the inhibition of viral replication. The three known members of this gene family are located in a cluster on chromosome 12. Mutations in this gene have been associated with host susceptibility to viral infection. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:P00973
NCBI GenInfo Identifier:296439492
NCBI Gene ID:4938
NCBI Accession:P00973.4
UniProt Secondary Accession:P00973,P04820, P29080, P29081, P78485, P78486, Q16700 Q16701, Q1PG42, Q3ZM01, Q53GC5, A8K4N8,
UniProt Related Accession:P00973
Molecular Weight:400
NCBI Full Name:2'-5'-oligoadenylate synthase 1
NCBI Synonym Full Names:2'-5'-oligoadenylate synthetase 1, 40/46kDa
NCBI Official Symbol:OAS1  
NCBI Official Synonym Symbols:OIAS; IFI-4; OIASI  
NCBI Protein Information:2'-5'-oligoadenylate synthase 1; E18/E16; p46/p42 OAS; 2-5A synthase 1; 2-5A synthetase 1; (2-5')oligo(A) synthase 1; 2',5'-oligo A synthetase 1; (2-5')oligo(A) synthetase 1; 2'-5'-oligoisoadenylate synthetase 1; 2',5'-oligoadenylate synthetase 1, 40/46kDa; 2'-5' oligoadenylate synthetase 1 p48 isoform; 2'-5' oligoadenylate synthetase 1 p52 isoform
UniProt Protein Name:2'-5'-oligoadenylate synthase 1
UniProt Synonym Protein Names:E18/E16; p46/p42 OAS
Protein Family:2'-5'-oligoadenylate synthase
UniProt Gene Name:OAS1  
UniProt Entry Name:OAS1_HUMAN
Anti-OAS1 Antibody (CAB14623)Western blot analysis of extracts of various cell lines, using OAS1 antibody (CAB14623) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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