Cell Biology Antibodies 9
Anti-NVL Antibody (CAB3995)
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- SKU:
- CAB3995
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-NVL Antibody |
| Antibody SKU: | CAB3995 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 340-520 of human NVL (NP_001230076.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | NCI-H460, MCF7, Mouse testis, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 340-520 of human NVL (NP_001230076.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | CLGI PDEA SRER ILQT LCRK LRLP QAFD FCHL AHLT PGFV GADL MALC REAA MCAV NRVL MKLQ EQQK KNPE MEDL PSKG VQEE RLGT EPTS ETQD ELQR LLGL LRDQ DPLS EEQM QGLC IELN DFIV ALSS VQPS AKRE GFVT VPNV TWAD IGAL EDIR EELT MAIL APVR NPDQ FKAL G |
| Gene ID: | 4931 |
| Uniprot: | O15381 |
| Cellular Location: | Nucleus, nucleolus, nucleoplasm |
| Calculated MW: | 72kDa/73kDa/82kDa/85kDa/95kDa |
| Observed MW: | 110kDa |
| Synonyms: | NVL, NVL2 |
| Background: | This gene encodes a member of the AAA (ATPases associated with diverse cellular activities) superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. Two encoded proteins, described as major and minor isoforms, have been localized to distinct regions of the nucleus. The largest encoded protein (major isoform) has been localized to the nucleolus and shown to participate in ribosome biosynthesis (PMID: 15469983, 16782053), while the minor isoform has been localized to the nucleoplasmin. |
| UniProt Protein Function: | NVL: a member of the AAA (ATPases associated with diverse cellular activities) superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. Two encoded proteins, described as major and minor isoforms, have been localized to distinct regions of the nucleus. The largest encoded protein (major isoform) has been localized to the nucleolus and shown to participate in ribosome biosynthesis (PMID: 15469983, 16782053), while the minor isoform has been localized to the nucleoplasmin. [provided by RefSeq, Aug 2011] |
| UniProt Protein Details: | Protein type:Nucleolus Chromosomal Location of Human Ortholog: 1q41-q42.2 Cellular Component: mitochondrion; membrane; cytoplasm; nucleolus; plasma membrane; nucleus Molecular Function:ATP binding |
| NCBI Summary: | This gene encodes a member of the AAA (ATPases associated with diverse cellular activities) superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. Two encoded proteins, described as major and minor isoforms, have been localized to distinct regions of the nucleus. The largest encoded protein (major isoform) has been localized to the nucleolus and shown to participate in ribosome biosynthesis (PMID: 15469983, 16782053), while the minor isoform has been localized to the nucleoplasmin. [provided by RefSeq, Aug 2011] |
| UniProt Code: | O15381 |
| NCBI GenInfo Identifier: | 32699415 |
| NCBI Gene ID: | 4931 |
| NCBI Accession: | O15381.1 |
| UniProt Secondary Accession: | O15381,Q96EM7, B4DMC4, B4DP98, |
| UniProt Related Accession: | O15381 |
| Molecular Weight: | 856 |
| NCBI Full Name: | Nuclear valosin-containing protein-like |
| NCBI Synonym Full Names: | nuclear VCP-like |
| NCBI Official Symbol: | NVL |
| NCBI Protein Information: | nuclear valosin-containing protein-like; NVLp |
| UniProt Protein Name: | Nuclear valosin-containing protein-like |
| Protein Family: | Nuclear valosin-containing protein |
| UniProt Gene Name: | NVL |
| UniProt Entry Name: | NVL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NVL antibody (CAB3995) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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