Cell Cycle Antibodies 1

Anti-NUSAP1 Antibody (CAB16000)

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SKU:
CAB16000
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-NUSAP1 Antibody
Antibody SKU:CAB16000
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-61 of human NUSAP1 (NP_060924.4).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-61 of human NUSAP1 (NP_060924.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MIIP SLEE LDSL KYSD LQNL AKSL GLRA NLRA TKLL KALK GYIK HEAR KGNE NQDE SQTS A
Gene ID:51203
Uniprot:Q9BXS6
Cellular Location:
Calculated MW:42kDa/47kDa/49kDa
Observed MW:49kDa
Synonyms:NUSAP1, ANKT, BM037, LNP, NUSAP, PRO0310p1, Q0310, SAPL
Background:NUSAP1 is a nucleolar-spindle-associated protein that plays a role in spindle microtubule organization (Raemaekers et al., 2003 [PubMed 12963707]).
UniProt Protein Function:NUSAP1: Microtubule-associated protein with the capacity to bundle and stabilize microtubules. May associate with chromosomes and promote the organization of mitotic spindle microtubules around them. Belongs to the NUSAP family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell cycle regulation; Nucleolus

Chromosomal Location of Human Ortholog: 15q15.1

Cellular Component: cytoplasm; nucleolus; nucleus

Molecular Function:protein binding

Biological Process: cytokinesis after mitosis; establishment of mitotic spindle localization; mitotic chromosome condensation; mitotic sister chromatid segregation; positive regulation of mitosis

NCBI Summary:NUSAP1 is a nucleolar-spindle-associated protein that plays a role in spindle microtubule organization (Raemaekers et al., 2003 [PubMed 12963707]).[supplied by OMIM, Jun 2009]
UniProt Code:Q9BXS6
NCBI GenInfo Identifier:74717631
NCBI Gene ID:51203
NCBI Accession:Q9BXS6.1
UniProt Secondary Accession:Q9BXS6,Q53GW2, Q8TBT4, Q96E58, Q96FJ1, Q9GZM9, Q9NZ85 Q9UI70, B4DDF1, E7ERR5, J3KN21,
UniProt Related Accession:Q9BXS6
Molecular Weight:42,352 Da
NCBI Full Name:Nucleolar and spindle-associated protein 1
NCBI Synonym Full Names:nucleolar and spindle associated protein 1
NCBI Official Symbol:NUSAP1  
NCBI Official Synonym Symbols:LNP; ANKT; SAPL; BM037; NUSAP; Q0310; PRO0310p1  
NCBI Protein Information:nucleolar and spindle-associated protein 1
UniProt Protein Name:Nucleolar and spindle-associated protein 1
Protein Family:Nucleolar and spindle-associated protein
UniProt Gene Name:NUSAP1  
UniProt Entry Name:NUSAP_HUMAN
Anti-NUSAP1 Antibody (CAB16000)Western blot analysis of extracts of various cell lines, using NUSAP1 antibody (CAB16000) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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