Cell Cycle Antibodies 1
Anti-NUSAP1 Antibody (CAB16000)
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- SKU:
- CAB16000
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-NUSAP1 Antibody |
| Antibody SKU: | CAB16000 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-61 of human NUSAP1 (NP_060924.4). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-61 of human NUSAP1 (NP_060924.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MIIP SLEE LDSL KYSD LQNL AKSL GLRA NLRA TKLL KALK GYIK HEAR KGNE NQDE SQTS A |
| Gene ID: | 51203 |
| Uniprot: | Q9BXS6 |
| Cellular Location: | |
| Calculated MW: | 42kDa/47kDa/49kDa |
| Observed MW: | 49kDa |
| Synonyms: | NUSAP1, ANKT, BM037, LNP, NUSAP, PRO0310p1, Q0310, SAPL |
| Background: | NUSAP1 is a nucleolar-spindle-associated protein that plays a role in spindle microtubule organization (Raemaekers et al., 2003 [PubMed 12963707]). |
| UniProt Protein Function: | NUSAP1: Microtubule-associated protein with the capacity to bundle and stabilize microtubules. May associate with chromosomes and promote the organization of mitotic spindle microtubules around them. Belongs to the NUSAP family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Nucleolus Chromosomal Location of Human Ortholog: 15q15.1 Cellular Component: cytoplasm; nucleolus; nucleus Molecular Function:protein binding Biological Process: cytokinesis after mitosis; establishment of mitotic spindle localization; mitotic chromosome condensation; mitotic sister chromatid segregation; positive regulation of mitosis |
| NCBI Summary: | NUSAP1 is a nucleolar-spindle-associated protein that plays a role in spindle microtubule organization (Raemaekers et al., 2003 [PubMed 12963707]).[supplied by OMIM, Jun 2009] |
| UniProt Code: | Q9BXS6 |
| NCBI GenInfo Identifier: | 74717631 |
| NCBI Gene ID: | 51203 |
| NCBI Accession: | Q9BXS6.1 |
| UniProt Secondary Accession: | Q9BXS6,Q53GW2, Q8TBT4, Q96E58, Q96FJ1, Q9GZM9, Q9NZ85 Q9UI70, B4DDF1, E7ERR5, J3KN21, |
| UniProt Related Accession: | Q9BXS6 |
| Molecular Weight: | 42,352 Da |
| NCBI Full Name: | Nucleolar and spindle-associated protein 1 |
| NCBI Synonym Full Names: | nucleolar and spindle associated protein 1 |
| NCBI Official Symbol: | NUSAP1 |
| NCBI Official Synonym Symbols: | LNP; ANKT; SAPL; BM037; NUSAP; Q0310; PRO0310p1 |
| NCBI Protein Information: | nucleolar and spindle-associated protein 1 |
| UniProt Protein Name: | Nucleolar and spindle-associated protein 1 |
| Protein Family: | Nucleolar and spindle-associated protein |
| UniProt Gene Name: | NUSAP1 |
| UniProt Entry Name: | NUSAP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NUSAP1 antibody (CAB16000) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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