Epigenetics & Nuclear Signaling Antibodies 2

Anti-NUR77 Antibody (CAB16264)

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SKU:
CAB16264
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-NUR77 Antibody
Antibody SKU:CAB16264
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200-300 of human NUR77 (NP_775180.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Rat
Positive Samples:Rat lung
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200-300 of human NUR77 (NP_775180.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PSLA QSPL KLFP SQAT HQLG EGES YSMP TAFP GLAP TSPH LEGS GILD TPVT STKA RSGA PGGS EGRC AVCG DNAS CQHY GVRT CEGC KGFF KRTV QKNA K
Gene ID:3164
Uniprot:P22736
Cellular Location:Cytoplasm, Nucleus
Calculated MW:33kDa/64kDa/65kDa
Observed MW:70kDa
Synonyms:NR4A1, GFRP1, HMR, N10, NAK-1, NGFIB, NP10, NUR77, TR3
Background:This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:Nur77: an orphan nuclear receptor and immediate-early gene that regulates cellular proliferation, apoptosis, inflammation, and glucose metabolism. Induced by exercise in muscle and is a functional regulator of glucose metabolism in skeletal muscle. Its level decreases in the muscle of obese insulin-resistant men. Acts concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. A mediator of TCR-directed thymocyte apoptosis. TCR-signaling induces a FAIM/Akt/Nur77 signaling pathway that is critical for modulating apoptosis in developing thymocytes. A physiological substrate of the MEK-ERK-RSK cascade that modulates nuclear export and intracellular translocation during T cell death. Binds DNA as a monomer. Interacts with GADD45GIP1. Overexpression of Nur77 induces the expression of both p300 and HDAC1. Acetylation by p300 and HDAC1 may regulate the rapid turnover of Nur77 protein.
UniProt Protein Details:

Protein type:Nuclear receptor; Apoptosis; DNA-binding

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nucleus

Molecular Function:protein binding; ligand-dependent nuclear receptor activity; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity

Biological Process: epidermal growth factor receptor signaling pathway; transcription initiation from RNA polymerase II promoter; phosphoinositide-mediated signaling; intracellular receptor-mediated signaling pathway; fibroblast growth factor receptor signaling pathway; cell migration during sprouting angiogenesis; nerve growth factor receptor signaling pathway; innate immune response; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; signal transduction

NCBI Summary:This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
UniProt Code:P22736
NCBI GenInfo Identifier:127819
NCBI Gene ID:3164
NCBI Accession:P22736.1
UniProt Secondary Accession:P22736,Q15627, B4DML7,
UniProt Related Accession:P22736
Molecular Weight:64,463 Da
NCBI Full Name:Nuclear receptor subfamily 4 group A member 1
NCBI Synonym Full Names:nuclear receptor subfamily 4, group A, member 1
NCBI Official Symbol:NR4A1  
NCBI Official Synonym Symbols:HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77  
NCBI Protein Information:nuclear receptor subfamily 4 group A member 1; ST-59; hormone receptor; TR3 orphan receptor; steroid receptor TR3; testicular receptor 3; early response protein NAK1; orphan nuclear receptor HMR; orphan nuclear receptor TR3; nuclear hormone receptor NUR/77; growth factor-inducible nuclear protein N10; nerve growth factor IB nuclear receptor variant 1
UniProt Protein Name:Nuclear receptor subfamily 4 group A member 1
UniProt Synonym Protein Names:Early response protein NAK1; Nuclear hormone receptor NUR/77; Nur77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; ST-59; Testicular receptor 3
Protein Family:Nuclear receptor subfamily
UniProt Gene Name:NR4A1  
UniProt Entry Name:NR4A1_HUMAN
Anti-NUR77 Antibody (CAB16264)Western blot analysis of extracts of rat lung, using NUR77 antibody (CAB16264) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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