Epigenetics & Nuclear Signaling Antibodies 1

Anti-NUR77 Antibody (CAB0843)

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SKU:
CAB0843
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-NUR77 Antibody
Antibody SKU:CAB0843
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human NUR77 (NP_775180.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:SH-SY5Y
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human NUR77 (NP_775180.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPCI QAQY GTPA PSPG PRDH LASD PLTP EFIK PTMD LASP EAAP AAPT ALPS FSTF MDGY TGEF DTFL YQLP GTVQ PCSS ASSS ASST SSSS ATSP ASAS FKFE DFQV YGCY PGPL SGPV DEAL SSSG SDYY GSPC SAPS PSTP SFQP PQLS PWDG SFGH FSPS QTYE GL
Gene ID:3164
Uniprot:P22736
Cellular Location:Cytoplasm, Nucleus
Calculated MW:33kDa/64kDa/65kDa
Observed MW:71kDa
Synonyms:NR4A1, GFRP1, HMR, N10, NAK-1, NGFIB, NP10, NUR77, TR3
Background:This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:Nur77: an orphan nuclear receptor and immediate-early gene that regulates cellular proliferation, apoptosis, inflammation, and glucose metabolism. Induced by exercise in muscle and is a functional regulator of glucose metabolism in skeletal muscle. Its level decreases in the muscle of obese insulin-resistant men. Acts concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. A mediator of TCR-directed thymocyte apoptosis. TCR-signaling induces a FAIM/Akt/Nur77 signaling pathway that is critical for modulating apoptosis in developing thymocytes. A physiological substrate of the MEK-ERK-RSK cascade that modulates nuclear export and intracellular translocation during T cell death. Binds DNA as a monomer. Interacts with GADD45GIP1. Overexpression of Nur77 induces the expression of both p300 and HDAC1. Acetylation by p300 and HDAC1 may regulate the rapid turnover of Nur77 protein.
UniProt Protein Details:

Protein type:Nuclear receptor; Apoptosis; DNA-binding

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nucleus

Molecular Function:protein binding; ligand-dependent nuclear receptor activity; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity

Biological Process: epidermal growth factor receptor signaling pathway; transcription initiation from RNA polymerase II promoter; phosphoinositide-mediated signaling; intracellular receptor-mediated signaling pathway; fibroblast growth factor receptor signaling pathway; cell migration during sprouting angiogenesis; nerve growth factor receptor signaling pathway; innate immune response; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; signal transduction

NCBI Summary:This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
UniProt Code:P22736
NCBI GenInfo Identifier:127819
NCBI Gene ID:3164
NCBI Accession:P22736.1
UniProt Secondary Accession:P22736,Q15627, B4DML7,
UniProt Related Accession:P22736
Molecular Weight:64,463 Da
NCBI Full Name:Nuclear receptor subfamily 4 group A member 1
NCBI Synonym Full Names:nuclear receptor subfamily 4, group A, member 1
NCBI Official Symbol:NR4A1  
NCBI Official Synonym Symbols:HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77  
NCBI Protein Information:nuclear receptor subfamily 4 group A member 1; ST-59; hormone receptor; TR3 orphan receptor; steroid receptor TR3; testicular receptor 3; early response protein NAK1; orphan nuclear receptor HMR; orphan nuclear receptor TR3; nuclear hormone receptor NUR/77; growth factor-inducible nuclear protein N10; nerve growth factor IB nuclear receptor variant 1
UniProt Protein Name:Nuclear receptor subfamily 4 group A member 1
UniProt Synonym Protein Names:Early response protein NAK1; Nuclear hormone receptor NUR/77; Nur77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; ST-59; Testicular receptor 3
Protein Family:Nuclear receptor subfamily
UniProt Gene Name:NR4A1  
UniProt Entry Name:NR4A1_HUMAN
Anti-NUR77 Antibody (CAB0843)Western blot analysis of extracts of SH-SY5Y cells, using NUR77 antibody (CAB0843) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-NUR77 Antibody (CAB0843)Immunofluorescence analysis of U2OS cells using NUR77 antibody (CAB0843). Blue: DAPI for nuclear staining.
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