Name: Anti-NUP85 Antibody (CAB11629)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB11629
Availability: OutOfStock

Antibody Name:	Anti-NUP85 Antibody
Antibody SKU:	CAB11629
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 427-656 of human NUP85 (NP_079120.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, U-87MG, A-549, LO2, HeLa, Mouse brain, Mouse testis, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 427-656 of human NUP85 (NP_079120.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	CPEL GRVS LELH IERI PLNT EQKA LKVL RICE QRQM TEQV RSIC KILA MKAV RNNR LGSA LSWS IRAK DAAF ATLV SDRF LRDY CERG CFSD LDLI DNLG PAMM LSDR LTFL GKYR EFHR MYGE KRFA DAAS LLLS LMTS RIAP RSFW MTLL TDAL PLLE QKQV IFSA EQTY ELMR CLED LTSR RPVH GESD TEQL QDDD IETT KVEM LRLS LARN LARA IIRE GSLE GS
Gene ID:	79902
Uniprot:	Q9BW27
Cellular Location:	Chromosome, Cytoplasm, Nucleus, Nucleus membrane, centromere, cytoskeleton, kinetochore, nuclear pore complex, spindle
Calculated MW:	52kDa/69kDa/75kDa
Observed MW:	70kDa

Synonyms:

NUP85, FROUNT, Nup75

Background:

This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. Nuclear pore complexes are embedded in the nuclear envelope and promote bidirectional transport of macromolecules between the cytoplasm and nucleus. The encoded protein can also bind to the C-terminus of chemokine (C-C motif) receptor 2 (CCR2) and promote chemotaxis of monocytes, thereby participating in the inflammatory response. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	NUP85: Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2- activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl- inositol 3-kinase-Rac-lammellipodium protrusion cascade. Belongs to the nucleoporin Nup85 family.
UniProt Protein Details:	Protein type:Nucleoporin Chromosomal Location of Human Ortholog: 17q25.1 Cellular Component: cytosol; kinetochore; membrane; nuclear envelope Molecular Function:protein binding; structural constituent of nuclear pore Biological Process: mitotic nuclear envelope disassembly; mRNA export from nucleus; positive regulation of transcription, DNA-dependent; protein import into nucleus; protein sumoylation; RNA-mediated gene silencing; sister chromatid cohesion; tRNA export from nucleus; viral reproduction; viral transcription
NCBI Summary:	This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. Nuclear pore complexes are embedded in the nuclear envelope and promote bidirectional transport of macromolecules between the cytoplasm and nucleus. The encoded protein can also bind to the C-terminus of chemokine (C-C motif) receptor 2 (CCR2) and promote chemotaxis of monocytes, thereby participating in the inflammatory response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
UniProt Code:	Q9BW27
NCBI GenInfo Identifier:	74733394
NCBI Gene ID:	79902
NCBI Accession:	Q9BW27.1
UniProt Secondary Accession:	Q9BW27,Q8NDI4, Q9H9U1, B4DMQ3, B4DPW1,
UniProt Related Accession:	Q9BW27
Molecular Weight:	69,800 Da
NCBI Full Name:	Nuclear pore complex protein Nup85
NCBI Synonym Full Names:	nucleoporin 85
NCBI Official Symbol:	NUP85
NCBI Official Synonym Symbols:	Nup75; FROUNT
NCBI Protein Information:	nuclear pore complex protein Nup85
UniProt Protein Name:	Nuclear pore complex protein Nup85
UniProt Synonym Protein Names:	85 kDa nucleoporin; FROUNT; Nucleoporin Nup75; Nucleoporin Nup85; Pericentrin-1
Protein Family:	Nucleoporin
UniProt Gene Name:	NUP85
UniProt Entry Name:	NUP85_HUMAN

Western blot analysis of extracts of various cell lines, using NUP85 antibody (CAB11629) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

Anti-NUP85 Antibody (CAB3504)

NUP85 Antibody (PACO10974)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)