Epigenetics & Nuclear Signaling Antibodies 2

Anti-NUP54 Antibody (CAB15454)

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SKU:
CAB15454
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-NUP54 Antibody
Antibody SKU:CAB15454
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human NUP54 (NP_059122.2).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, LO2, Mouse thymus, Mouse lung, Mouse testis, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human NUP54 (NP_059122.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LGGL FSQP TQAP TQSN QLIN TASA LSAP TLLG DERD AILA KWNQ LQAF WGTG KGYF NNNI PPVE FTQE NPFC RFKA VGYS CMPS NKDE DGLV VLVF NKKE TEIR SQQQ QLVE SLHK VLGG NQTL TVNV EGTK TLPD DQTE VVIY VVER SPNG TSRR VPAT TLYA HFEQ ANIK TQLQ QLGV TLSM TRTE LSPA QIKQ LLQN PPAG VDPI IWEQ AKVD NPDS EKLI PVPM VGFK ELLR RLKV QDQM TKQH QTRL DIIS EDIS ELQK NQTT SVAK IAQY KRKL MDLS HRTL QVLI KQEI QRKS G
Gene ID:53371
Uniprot:Q7Z3B4
Cellular Location:Cytoplasmic side, Nucleoplasmic side, Nucleus, Nucleus membrane, Peripheral membrane protein, nuclear pore complex
Calculated MW:33kDa/50kDa/55kDa
Observed MW:60kDa
Synonyms:NUP54
Background:The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. This gene encodes a member of the phe-gly (FG) repeat-containing nucleoporin subset. Multiple alternatively spliced transcript variants have been found for this gene.
NCBI Summary:The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. This gene encodes a member of the phe-gly (FG) repeat-containing nucleoporin subset. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013]
UniProt Code:Q7Z3B4
NCBI GenInfo Identifier:26051237
NCBI Gene ID:53371
NCBI Accession:NP_059122.2
UniProt Related Accession:Q7Z3B4
Molecular Weight:55 kDa
NCBI Full Name:nucleoporin p54 isoform 1
NCBI Synonym Full Names:nucleoporin 54
NCBI Official Symbol:NUP54  
NCBI Protein Information:nucleoporin p54
UniProt Protein Name:Nucleoporin p54
UniProt Synonym Protein Names:54 kDa nucleoporin
Protein Family:Nucleoporin
UniProt Gene Name:NUP54  
UniProt Entry Name:NUP54_HUMAN
Anti-NUP54 Antibody (CAB15454)Western blot analysis of extracts of various cell lines, using NUP54 antibody (CAB15454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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