Antibody Name:	Anti-NUP50 Antibody
Antibody SKU:	CAB9441
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 299-468 of human NUP50 (NP_009103.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 299-468 of human NUP50 (NP_009103.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NSSL FGKD TTQS KPVS SPFP TKPL EGQA EGDS GECK GGDE EEND EPPK VVVT EVKE EDAF YSKK CKLF YKKD NEFK EKGI GTLH LKPT ANQK TQLL VRAD TNLG NILL NVLI PPNM PCTR TGKN NVLI VCVP NPPI DEKN ATMP VTML IRVK TSED ADEL HKIL LEKK DA
Gene ID:	10762
Uniprot:	Q9UKX7
Cellular Location:	Nucleoplasmic side, Nucleus, Nucleus membrane, Peripheral membrane protein, nuclear pore complex
Calculated MW:	46kDa/50kDa
Observed MW:	58kDa

Synonyms:

NUP50, NPAP60, NPAP60L

Background:

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	NUP50: Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin- alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear import; Nucleoporin Chromosomal Location of Human Ortholog: 22q13.31 Cellular Component: cytoplasm; nuclear membrane; nucleoplasm Molecular Function:protein binding; Ran GTPase binding Biological Process: mitotic nuclear envelope disassembly; mRNA export from nucleus; protein import into nucleus; protein sumoylation; RNA-mediated gene silencing; tRNA export from nucleus; viral reproduction; viral transcription
NCBI Summary:	The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UKX7
NCBI GenInfo Identifier:	20455193
NCBI Gene ID:	10762
NCBI Accession:	Q9UKX7.2
UniProt Secondary Accession:	Q9UKX7,O75644, Q8N6V5, Q9NPM9, Q9NPR6, Q9P1K5, B1AHA4 B2RB15,
UniProt Related Accession:	Q9UKX7
Molecular Weight:	46,863 Da
NCBI Full Name:	Nuclear pore complex protein Nup50
NCBI Synonym Full Names:	nucleoporin 50
NCBI Official Symbol:	NUP50
NCBI Official Synonym Symbols:	NPAP60; NPAP60L
NCBI Protein Information:	nuclear pore complex protein Nup50
UniProt Protein Name:	Nuclear pore complex protein Nup50
UniProt Synonym Protein Names:	50 kDa nucleoporin; Nuclear pore-associated protein 60 kDa-like; Nucleoporin Nup50
Protein Family:	Nuclear pore complex protein
UniProt Gene Name:	NUP50
UniProt Entry Name:	NUP50_HUMAN

	Western blot analysis of extracts of various cell lines, using NUP50 antibody (CAB9441) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat testis using NUP50 Rabbit pAb (CAB9441) at dilution of 1:100 (40x lens).