Epigenetics & Nuclear Signaling Antibodies 1

Anti-NUP35 Antibody (CAB12762)

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SKU:
CAB12762
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-NUP35 Antibody
Antibody SKU:CAB12762
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human NUP35 (NP_612142.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A-431, HeLa, Jurkat, LO2, Mouse brain, Mouse pancreas, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human NUP35 (NP_612142.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAF AVEP QGPA LGSE PMML GSPT SPKP GVNA QFLP GFLM GDLP APVT PQPR SISG PSVG VMEM RSPL LAGG SPPQ PVVP AHKD KSGA PPVR SIYD DISS PGLG STPL TSRR QPNI SVMQ SPLV GVTS TPGT GQSM FSPA SIGQ PRKT TLSP AQLD PFYT QGDS LTSE DHLD DSWV TVFG FPQA SASY ILLQ FAQY GNIL KHVM SNTG NWMH IRYQ SKLQ ARKA LSKD GRIF GESI MIGV KPCI DKSV MESS DRCA LSSP SLAF TPPI KTLG TPTQ PGST PRIS TMRP LATA YKAS TSDY QVIS DRQT PKKD ESLV SKAM EYMF GW
Gene ID:129401
Uniprot:Q8NFH5
Cellular Location:Nucleus, Nucleus membrane, Peripheral membrane protein, nuclear pore complex
Calculated MW:21kDa/33kDa/34kDa
Observed MW:37kDa
Synonyms:NUP35, MP-44, MP44, NP44, NUP53
Background:This gene encodes a member of the nucleoporin family. The encoded protein contains two membrane binding regions, is localized to the nuclear rim, and is part of the nuclear pore complex. All molecules entering or leaving the nucleus either diffuse through or are actively transported by the nuclear pore complex. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 7 and 10.
UniProt Protein Function:NUP35: Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs). Can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC. Belongs to the Nup53 family.
UniProt Protein Details:

Protein type:Nucleoporin

Chromosomal Location of Human Ortholog: 2q32.1

Cellular Component: nuclear envelope; nuclear membrane; nucleoplasm; plasma membrane

Molecular Function:nucleocytoplasmic transporter activity; phospholipid binding; protein binding; single-stranded DNA binding

Biological Process: mitotic nuclear envelope disassembly; mRNA export from nucleus; NLS-bearing substrate import into nucleus; nuclear pore organization and biogenesis; protein sumoylation; regulation of transcription, DNA-dependent; RNA-mediated gene silencing; tRNA export from nucleus; viral reproduction; viral transcription

NCBI Summary:This gene encodes a member of the nucleoporin family. The encoded protein contains two membrane binding regions, is localized to the nuclear rim, and is part of the nuclear pore complex. All molecules entering or leaving the nucleus either diffuse through or are actively transported by the nuclear pore complex. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 7 and 10. [provided by RefSeq, Dec 2013]
UniProt Code:Q8NFH5
NCBI GenInfo Identifier:74730292
NCBI Gene ID:129401
NCBI Accession:Q8NFH5.1
UniProt Secondary Accession:Q8NFH5,Q4ZFZ9, Q53S95, Q8TDJ1, B4DP57, B4DYB4,
UniProt Related Accession:Q8NFH5
Molecular Weight:35kDa
NCBI Full Name:Nucleoporin NUP53
NCBI Synonym Full Names:nucleoporin 35
NCBI Official Symbol:NUP35  
NCBI Official Synonym Symbols:MP44; NP44; MP-44; NUP53  
NCBI Protein Information:nucleoporin NUP53
UniProt Protein Name:Nucleoporin NUP53
UniProt Synonym Protein Names:35 kDa nucleoporin; Mitotic phosphoprotein 44; MP-44; Nuclear pore complex protein Nup53; Nucleoporin Nup35
Protein Family:Nucleoporin
UniProt Gene Name:NUP35  
Anti-NUP35 Antibody (CAB12762)Western blot analysis of extracts of various cell lines, using NUP35 antibody (CAB12762) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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