Immunology Antibodies 3

Anti-NUP214 Antibody (CAB8357)

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SKU:
CAB8357
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology

Description

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Antibody Name:Anti-NUP214 Antibody
Antibody SKU:CAB8357
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 750-1030 of human NUP214 (NP_005076.3).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, 293T, Mouse brain, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 750-1030 of human NUP214 (NP_005076.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FLLE IKET TESL HGDI SSLK TTLL EGFA GVEE AREQ NERN RDSG YLHL LYKR PLDP KSEA QLQE IRRL HQYV KFAV QDVN DVLD LEWD QHLE QKKK QRHL LVPE RETL FNTL ANNR EIIN QQRK RLNH LVDS LQQL RLYK QTSL WSLS SAVP SQSS IHSF DSDL ESLC NALL KTTI ESHT KSLP KVPA KLSP MKQA QLRN FLAK RKTP PVRS TAPA SLSR SAFL SQRY YEDL DEVS STSS VSQS LESE DART SCKD DEAV VQAP RHAP VVRT PSIQ PSLL P
Gene ID:8021
Uniprot:P35658
Cellular Location:Nucleus, nuclear pore complex
Calculated MW:212kDa/213kDa/215kDa
Observed MW:251kDa
Synonyms:NUP214, CAIN, CAN
Background:The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This gene is a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for proper cell cycle progression and nucleocytoplasmic transport. The 3' portion of this gene forms a fusion gene with the DEK gene on chromosome 6 in a t(6, 9) translocation associated with acute myeloid leukemia and myelodysplastic syndrome. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:NUP214: May serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex. A chromosomal aberration involving NUP214 is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia. Translocation t(6;9)(p23;q34) with DEK. It results in the formation of a DEK-CAN fusion gene. A chromosomal aberration involving NUP214 is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with SET. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Oncoprotein; Nucleoporin; Nuclear export

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: nucleoplasm; focal adhesion; intracellular membrane-bound organelle; nuclear pore; nucleus; cytosol

Molecular Function:protein binding; transporter activity; nucleocytoplasmic transporter activity

Biological Process: viral reproduction; regulation of cell cycle; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; viral infectious cycle; glucose transport; mRNA export from nucleus; protein import into nucleus; hexose transport; carbohydrate metabolic process; viral transcription; gene expression; mitotic cell cycle; protein export from nucleus; transmembrane transport

Disease: Leukemia, Acute Lymphoblastic

NCBI Summary:The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This gene is a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for proper cell cycle progression and nucleocytoplasmic transport. The 3' portion of this gene forms a fusion gene with the DEK gene on chromosome 6 in a t(6,9) translocation associated with acute myeloid leukemia and myelodysplastic syndrome. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
UniProt Code:P35658
NCBI GenInfo Identifier:33946327
NCBI Gene ID:8021
NCBI Accession:NP_005076.3
UniProt Related Accession:P35658
Molecular Weight:
NCBI Full Name:nuclear pore complex protein Nup214 isoform 2
NCBI Synonym Full Names:nucleoporin 214
NCBI Official Symbol:NUP214  
NCBI Official Synonym Symbols:CAN; CAIN  
NCBI Protein Information:nuclear pore complex protein Nup214
UniProt Protein Name:Nuclear pore complex protein Nup214
UniProt Synonym Protein Names:214 kDa nucleoporin; Nucleoporin Nup214; Protein CAN
UniProt Gene Name:NUP214  
UniProt Entry Name:NU214_HUMAN
Anti-NUP214 Polyclonal Antibody (CAB8357)Western blot analysis of extracts of various cell lines, using NUP214 antibody (CAB8357) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-NUP214 Polyclonal Antibody (CAB8357)Immunofluorescence analysis of L929 cells using Anti-NUP214 Polyclonal Antibody (CAB8357) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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