Epigenetics & Nuclear Signaling Antibodies 4

Anti-NUP133 Antibody (CAB8818)

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SKU:
CAB8818
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-NUP133 Antibody
Antibody SKU:CAB8818
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 730-930 of human NUP133 (NP_060700.2).
Application:WB IF
Recommended Dilution:WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:A375, LO2, HeLa, NCI-H460
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 730-930 of human NUP133 (NP_060700.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NILK DMLQ AASH YRQN RNSL YRRE ESLE KEPE YVPW TATS GPGG IRTV IIRQ HEIV LKVA YPQA DSNL RNIV TEQL VALI DCFL DGYV SQLK SVDK SSNR ERYD NLEM EYLQ KRSD LLSP LLSL GQYL WAAS LAEK YCDF DILV QMCE QTDN QSRL QRYM TQFA DQNF SDFL FRWY LEKG KRGK LLSQ PISQ HGQL ANFL Q
Gene ID:55746
Uniprot:Q8WUM0
Cellular Location:Chromosome, Nucleus, centromere, kinetochore, nuclear pore complex
Calculated MW:128kDa
Observed MW:133kDa
Synonyms:NUP133, hNUP133
Background:The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.
NCBI Summary:The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells. [provided by RefSeq, Jul 2008]
UniProt Code:Q8WUM0
NCBI GenInfo Identifier:26051235
NCBI Gene ID:55746
NCBI Accession:NP_060700.2
UniProt Related Accession:Q8WUM0
Molecular Weight:
NCBI Full Name:nuclear pore complex protein Nup133
NCBI Synonym Full Names:nucleoporin 133
NCBI Official Symbol:NUP133  
NCBI Official Synonym Symbols:GAMOS8; NPHS18; hNUP133  
NCBI Protein Information:nuclear pore complex protein Nup133
UniProt Protein Name:Nuclear pore complex protein Nup133
UniProt Synonym Protein Names:133 kDa nucleoporin; Nucleoporin Nup133
UniProt Gene Name:NUP133  
UniProt Entry Name:NU133_HUMAN
Anti-NUP133 Polyclonal Antibody (CAB8818)Western blot analysis of extracts of various cell lines, using NUP133 antibody (CAB8818) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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