Neuroscience
Anti-NUMB Antibody (CAB9352)
- SKU:
- CAB9352
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Neuroscience
Description
| Antibody Name: | Anti-NUMB Antibody |
| Antibody SKU: | CAB9352 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 521-640 of human NUMB (NP_003735.3). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, LO2, U-87MG, HT-1080, SW480, A-549, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 521-640 of human NUMB (NP_003735.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SQMV ANVF GTAG HPQA AHPH QSPS LVRQ QTFP HYEA SSAT TSPF FKPP AQHL NGSA AFNG VDDG RLAS ADRH TEVP TGTC PVDP FEAQ WAAL ENKS KQRT NPSP TNPF SSDL QKTF EIEL |
| Gene ID: | 8650 |
| Uniprot: | P49757 |
| Cellular Location: | Membrane, Peripheral membrane protein |
| Calculated MW: | 37kDa/48-70kDa |
| Observed MW: | 71kDa |
| Synonyms: | NUMB, C14orf41, S171, c14_5527 |
| Background: | The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | NUMB: Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. Interacts with CDH1 and TFAP2B. Interacts with EPS15, LNX and NOTCH1. May interact with DUOXA1. Interacts with RALBP1 in a complex also containing EPN1 and TFAP2A during interphase and mitosis. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell development/differentiation Chromosomal Location of Human Ortholog: 14q24.3 Cellular Component: clathrin-coated vesicle; focal adhesion; plasma membrane Molecular Function:protein binding Biological Process: lateral ventricle development; neuroblast division in the subventricular zone; positive regulation of cell migration; positive regulation of neurogenesis |
| NCBI Summary: | The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
| UniProt Code: | P49757 |
| NCBI GenInfo Identifier: | 14195675 |
| NCBI Gene ID: | 8650 |
| NCBI Accession: | P49757.2 |
| UniProt Secondary Accession: | P49757,Q6NUQ7, Q86SY1, Q8WW73, Q9UBG1, Q9UEQ4, B1P2N5 B1P2N6, B1P2N7, B1P2N8, B1P2N9, B4E2B1, |
| UniProt Related Accession: | P49757 |
| Molecular Weight: | 37,396 Da |
| NCBI Full Name: | Protein numb homolog |
| NCBI Synonym Full Names: | NUMB, endocytic adaptor protein |
| NCBI Official Symbol: | NUMB |
| NCBI Official Synonym Symbols: | S171; C14orf41; c14_5527 |
| NCBI Protein Information: | protein numb homolog |
| UniProt Protein Name: | Protein numb homolog |
| UniProt Synonym Protein Names: | Protein S171 |
| Protein Family: | Numb-like protein |
| UniProt Gene Name: | NUMB |
| UniProt Entry Name: | NUMB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NUMB antibody (CAB9352) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of L929 cells using NUMB antibody (CAB9352) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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