Name: Anti-NUMB Antibody (CAB9352)
Brand: Neuroscience
SKU: CAB9352
Availability: OutOfStock

Antibody Name:	Anti-NUMB Antibody
Antibody SKU:	CAB9352
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 521-640 of human NUMB (NP_003735.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, LO2, U-87MG, HT-1080, SW480, A-549, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 521-640 of human NUMB (NP_003735.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SQMV ANVF GTAG HPQA AHPH QSPS LVRQ QTFP HYEA SSAT TSPF FKPP AQHL NGSA AFNG VDDG RLAS ADRH TEVP TGTC PVDP FEAQ WAAL ENKS KQRT NPSP TNPF SSDL QKTF EIEL
Gene ID:	8650
Uniprot:	P49757
Cellular Location:	Membrane, Peripheral membrane protein
Calculated MW:	37kDa/48-70kDa
Observed MW:	71kDa

Synonyms:	NUMB, C14orf41, S171, c14_5527
Background:	The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	NUMB: Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. Interacts with CDH1 and TFAP2B. Interacts with EPS15, LNX and NOTCH1. May interact with DUOXA1. Interacts with RALBP1 in a complex also containing EPN1 and TFAP2A during interphase and mitosis. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell development/differentiation Chromosomal Location of Human Ortholog: 14q24.3 Cellular Component: clathrin-coated vesicle; focal adhesion; plasma membrane Molecular Function:protein binding Biological Process: lateral ventricle development; neuroblast division in the subventricular zone; positive regulation of cell migration; positive regulation of neurogenesis
NCBI Summary:	The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
UniProt Code:	P49757
NCBI GenInfo Identifier:	14195675
NCBI Gene ID:	8650
NCBI Accession:	P49757.2
UniProt Secondary Accession:	P49757,Q6NUQ7, Q86SY1, Q8WW73, Q9UBG1, Q9UEQ4, B1P2N5 B1P2N6, B1P2N7, B1P2N8, B1P2N9, B4E2B1,
UniProt Related Accession:	P49757
Molecular Weight:	37,396 Da
NCBI Full Name:	Protein numb homolog
NCBI Synonym Full Names:	NUMB, endocytic adaptor protein
NCBI Official Symbol:	NUMB
NCBI Official Synonym Symbols:	S171; C14orf41; c14_5527
NCBI Protein Information:	protein numb homolog
UniProt Protein Name:	Protein numb homolog
UniProt Synonym Protein Names:	Protein S171
Protein Family:	Numb-like protein
UniProt Gene Name:	NUMB
UniProt Entry Name:	NUMB_HUMAN

	Western blot analysis of extracts of various cell lines, using NUMB antibody (CAB9352) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of L929 cells using NUMB antibody (CAB9352) at dilution of 1:100. Blue: DAPI for nuclear staining.