Cell Cycle Antibodies 1

Anti-NUMA1 Antibody (CAB0527)

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SKU:
CAB0527
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-NUMA1 Antibody
Antibody SKU:CAB0527
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human NUMA1 (NP_006176.2).
Application:WB IHC IF
Recommended Dilution:WB 1:200 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human NUMA1 (NP_006176.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTLH ATRG AALL SWVN SLHV ADPV EAVL QLQD CSIF IKII DRIH GTEE GQQI LKQP VSER LDFV CSFL QKNR KHPS SPEC LVSA QKVL EGSE LELA KMTM LLLY HSTM SSKS PRDW EQFE YKIQ AELA VILK FVLD HEDG LNLN EDLE NFLQ KAPV PSTC SSTF PEEL SPPS HQAK REIR FLEL QKVA SSSS GNNF LSGS PASP MGDI LQTP QFQM RRLK KQLA DERS NRDE LELE LAEN RKLL TEKD AQIA MMQQ RIDR LALL NEKQ AASP LEPK ELEE LRDK NESL TMRL HETL KQCQ DLKT EKSQ M
Gene ID:4926
Uniprot:Q14980
Cellular Location:Chromosome, Cytoplasm, Nucleus matrix, cytoskeleton, spindle pole
Calculated MW:109kDa/200kDa/201kDa/236kDa/238kDa
Observed MW:238kDa
Synonyms:NUMA1, NMP-22, NUMA
Background:This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:NuMA-1: a coiled-coil nuclear protein that is required to establish and maintain mammalian spindle poles. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident. Two human splice-variant isoforms have been described.
UniProt Protein Details:

Protein type:Cell cycle regulation; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: spindle pole; nuclear matrix; dendrite; chromosome; cytosol; nucleoplasm; Golgi membrane; cell soma; apical part of cell; spindle microtubule; cytoplasm; spindle; nucleus

Molecular Function:protein binding; microtubule binding; structural molecule activity

Biological Process: mitosis; meiotic cell cycle; establishment of mitotic spindle orientation; cell division; mitotic cell cycle; G2/M transition of mitotic cell cycle; nuclear organization and biogenesis

Disease: Acute Promyelocytic Leukemia

NCBI Summary:This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
UniProt Code:Q14980
NCBI GenInfo Identifier:145559510
NCBI Gene ID:4926
NCBI Accession:Q14980.2
UniProt Secondary Accession:Q14980,Q14981, Q9BTE9, H0YH75,
UniProt Related Accession:Q14980
Molecular Weight:109,279 Da
NCBI Full Name:Nuclear mitotic apparatus protein 1
NCBI Synonym Full Names:nuclear mitotic apparatus protein 1
NCBI Official Symbol:NUMA1  
NCBI Official Synonym Symbols:NUMA  
NCBI Protein Information:nuclear mitotic apparatus protein 1; SP-H antigen; structural nuclear protein; centrophilin stabilizes mitotic spindle in mitotic cells
UniProt Protein Name:Nuclear mitotic apparatus protein 1
UniProt Synonym Protein Names:Nuclear matrix protein-22
Protein Family:Nuclear mitotic apparatus protein
UniProt Gene Name:NUMA1  
UniProt Entry Name:NUMA1_HUMAN
Anti-NUMA1 Antibody (CAB0527)Western blot analysis of extracts of various cell lines, using NUMA1 antibody (CAB0527) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-NUMA1 Antibody (CAB0527)Immunohistochemistry of paraffin-embedded human stomach using NUMA1 antibody (CAB0527) at dilution of 1:100 (40x lens).
Anti-NUMA1 Antibody (CAB0527)Immunohistochemistry of paraffin-embedded rat heart using NUMA1 antibody (CAB0527) at dilution of 1:100 (40x lens).
Anti-NUMA1 Antibody (CAB0527)Immunohistochemistry of paraffin-embedded mouse stomach using NUMA1 antibody (CAB0527) at dilution of 1:100 (40x lens).
Anti-NUMA1 Antibody (CAB0527)Immunofluorescence analysis of U2OS cells using NUMA1 antibody (CAB0527) at dilution of 1:100. Blue: DAPI for nuclear staining.
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