Antibody Name:	Anti-NUDT15 Antibody
Antibody SKU:	CAB8368
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human NUDT15 (NP_060753.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, Raji

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human NUDT15 (NP_060753.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTAS AQPR GRRP GVGV GVVV TSCK HPRC VLLG KRKG SVGA GSFQ LPGG HLEF GETW EECA QRET WEEA ALHL KNVH FASV VNSF IEKE NYHY VTIL MKGE VDVT HDSE PKNV EPEK NESW EWVP WEEL PPLD QLFW GLRC LKEQ GYDP FKED LNHL VGYK GNHL
Gene ID:	55270
Uniprot:	Q9NV35
Cellular Location:
Calculated MW:	18kDa
Observed MW:	19kDa

Synonyms:

NUDT15, MTH2, NUDT15D

Background:

This gene encodes an enzyme that belongs to the Nudix hydrolase superfamily. Members of this superfamily catalyze the hydrolysis of nucleoside diphosphates, including substrates like 8-oxo-dGTP, which are a result of oxidative damage, and can induce base mispairing during DNA replication, causing transversions. The encoded enzyme is a negative regulator of thiopurine activation and toxicity. Mutations in this gene result in poor metabolism of thiopurines, and are associated with thiopurine-induced early leukopenia. Multiple pseudogenes of this gene have been identified.

UniProt Protein Function:	NUDT15: Mediates the hydrolysis of some nucleoside diphosphate derivatives. Can degrade 8-oxo-dGTP in vitro, suggesting that it may remove an oxidatively damaged form of guanine (7,8-dihydro-8- oxoguanine) from DNA and the nucleotide pool, thereby preventing misincorporation of 8-oxo-dGTP into DNA thus preventing A:T to C:G transversions. Its substrate specificity in vivo however remains unclear. May have a role in DNA synthesis and cell cycle progression through the interaction with PCNA. Belongs to the Nudix hydrolase family.Protein type: Hydrolase; EC 3.6.1.55Chromosomal Location of Human Ortholog: 13q14.2Cellular Component: cytosolMolecular Function: 8-oxo-7,8-dihydroguanine triphosphatase activity; nucleoside-diphosphatase activityBiological Process: dGTP catabolic process
UniProt Protein Details:
NCBI Summary:	This gene encodes an enzyme that belongs to the Nudix hydrolase superfamily. Members of this superfamily catalyze the hydrolysis of nucleoside diphosphates, including substrates like 8-oxo-dGTP, which are a result of oxidative damage, and can induce base mispairing during DNA replication, causing transversions. The encoded enzyme is a negative regulator of thiopurine activation and toxicity. Mutations in this gene result in poor metabolism of thiopurines, and are associated with thiopurine-induced early leukopenia. Multiple pseudogenes of this gene have been identified. [provided by RefSeq, Apr 2016]
UniProt Code:	Q9NV35
NCBI GenInfo Identifier:	68565944
NCBI Gene ID:	55270
NCBI Accession:	Q9NV35.1
UniProt Secondary Accession:	Q9NV35,Q32Q27, Q6P2C9, A2RUR6
UniProt Related Accession:	Q9NV35
Molecular Weight:	18,609 Da
NCBI Full Name:	Probable 8-oxo-dGTP diphosphatase NUDT15
NCBI Synonym Full Names:	nudix hydrolase 15
NCBI Official Symbol:	NUDT15
NCBI Official Synonym Symbols:	MTH2; NUDT15D
NCBI Protein Information:	probable 8-oxo-dGTP diphosphatase NUDT15
UniProt Protein Name:	Probable 8-oxo-dGTP diphosphatase NUDT15
UniProt Synonym Protein Names:	7,8-dihydro-8-oxoguanine-triphosphatase NUDT15; MutT homolog 2; MTH2; Nucleoside diphosphate-linked moiety X motif 15; Nudix motif 15
Protein Family:	Nudix hydrolase
UniProt Gene Name:	NUDT15
UniProt Entry Name:	NUD15_HUMAN

	Western blot analysis of extracts of various cell lines, using NUDT15 antibody (CAB8368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver using NUDT15 antibody (CAB8368) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using NUDT15 antibody (CAB8368) at dilution of 1:100 (40x lens).