Cell Biology Antibodies 9
Anti-NUDT1 Antibody (CAB5474)
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- SKU:
- CAB5474
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-NUDT1 Antibody |
| Antibody SKU: | CAB5474 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human NUDT1 (NP_945192.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | SW480, K-562, 22Rv1, THP-1, HepG2, SKOV3, 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human NUDT1 (NP_945192.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSGI SPQQ MGEP EGSW SGKN PGTM GASR LYTL VLVL QPQR VLLG MKKR GFGA GRWN GFGG KVQE GETI EDGA RREL QEES GLTV DALH KVGQ IVFE FVGE PELM DVHV FCTD SIQG TPVE SDEM RPCW FQLD QIPF KDMW PDDS YWFP LLLQ KKKF HGYF KFQG QDTI LDYT LREV DTV |
| Gene ID: | 4521 |
| Uniprot: | P36639 |
| Cellular Location: | Cytoplasm, Mitochondrion matrix, Nucleus |
| Calculated MW: | 17kDa/19kDa/20kDa/22kDa |
| Observed MW: | 18kDa |
| Synonyms: | NUDT1, MTH1 |
| Background: | Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described. |
| UniProt Protein Function: | NUDT1: Antimutagenic. Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress. Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. Belongs to the Nudix hydrolase family. 4 isoforms of the human protein are produced by alternative initiation. |
| UniProt Protein Details: | Protein type:EC 3.6.1.55; Hydrolase; EC 3.6.1.56 Chromosomal Location of Human Ortholog: 7p22 Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; nucleus; cytosol Molecular Function:GTPase activity; snoRNA binding; m7G(5')pppN diphosphatase activity; metal ion binding; ATP diphosphatase activity; 8-oxo-7,8-dihydroguanine triphosphatase activity Biological Process: DNA protection; dGTP catabolic process; nucleobase, nucleoside and nucleotide metabolic process; dATP catabolic process; purine nucleotide catabolic process; response to oxidative stress; DNA repair |
| NCBI Summary: | Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P36639 |
| NCBI GenInfo Identifier: | 254763430 |
| NCBI Gene ID: | 4521 |
| NCBI Accession: | P36639.3 |
| UniProt Secondary Accession: | P36639,Q6LES7, Q6P0Y6, Q7Z7N6, Q8IV95, Q9UBM0, Q9UBM9 A4D205, |
| UniProt Related Accession: | P36639 |
| Molecular Weight: | 197 |
| NCBI Full Name: | 7,8-dihydro-8-oxoguanine triphosphatase |
| NCBI Synonym Full Names: | nudix (nucleoside diphosphate linked moiety X)-type motif 1 |
| NCBI Official Symbol: | NUDT1 |
| NCBI Official Synonym Symbols: | MTH1 |
| NCBI Protein Information: | 7,8-dihydro-8-oxoguanine triphosphatase; 8-oxo-dGTPase; nudix motif 1; mutT human homolog 1; 2-hydroxy-dATP diphosphatase; 8-oxo-7,8-dihydroguanosine triphosphatase; 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase; nucleoside diphosphate-linked moiety X motif 1; nucleoside diphosphate-linked moiety X-type motif 1 |
| UniProt Protein Name: | 7,8-dihydro-8-oxoguanine triphosphatase |
| UniProt Synonym Protein Names: | 2-hydroxy-dATP diphosphatase (EC:3.6.1.56); 8-oxo-dGTPase; Nucleoside diphosphate-linked moiety X motif 1; Nudix motif 1 |
| Protein Family: | Nudix hydrolase |
| UniProt Gene Name: | NUDT1 |
| UniProt Entry Name: | 8ODP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NUDT1 antibody (CAB5474) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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