Signal Transduction Antibodies 1
Anti-NUCB2 Antibody (CAB12642)
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- SKU:
- CAB12642
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-NUCB2 Antibody |
| Antibody SKU: | CAB12642 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 321-420 of human NUCB2 (NP_005004.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 321-420 of human NUCB2 (NP_005004.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ATEK KEFL EPDS WETL DQQQ FFTE EELK EYEN IIAL QENE LKKK ADEL QKQK EELQ RQHD QLEA QKLE YHQV IQQM EQKK LQQG IPPS GPAG ELKF EPHI |
| Gene ID: | 4925 |
| Uniprot: | P80303 |
| Cellular Location: | Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus envelope, Peripheral membrane protein, Secreted |
| Calculated MW: | 50kDa |
| Observed MW: | 50kDa |
| Synonyms: | NUCB2, HEL-S-109, NEFA |
| Background: | This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. |
| UniProt Protein Function: | NUCB2: Calcium-binding protein. May have a role in calcium homeostasis. Belongs to the nucleobindin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Vesicle; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 11p15.1 Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum; ER-Golgi intermediate compartment; plasma membrane; nuclear envelope; cytosol Molecular Function:protein binding; DNA binding; calcium ion binding |
| NCBI Summary: | This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011] |
| UniProt Code: | P80303 |
| NCBI GenInfo Identifier: | 224471846 |
| NCBI Gene ID: | 4925 |
| NCBI Accession: | P80303.2 |
| UniProt Secondary Accession: | P80303,Q8NFT5, A8K642, D3DQX5, |
| UniProt Related Accession: | P80303 |
| Molecular Weight: | 50,201 Da |
| NCBI Full Name: | Nucleobindin-2 |
| NCBI Synonym Full Names: | nucleobindin 2 |
| NCBI Official Symbol: | NUCB2 |
| NCBI Official Synonym Symbols: | NEFA; HEL-S-109 |
| NCBI Protein Information: | nucleobindin-2; nesfatin 1; nesfatin-1; prepronefastin; prepronesfatin; nucleobinding 2; DNA-binding protein NEFA; gastric cancer antigen Zg4; epididymis secretory protein Li 109 |
| UniProt Protein Name: | Nucleobindin-2 |
| UniProt Synonym Protein Names: | DNA-binding protein NEFA; Gastric cancer antigen Zg4; PrepronesfatinCleaved into the following chain:Nesfatin-1 |
| Protein Family: | Nucleobindin |
| UniProt Gene Name: | NUCB2 |
| UniProt Entry Name: | NUCB2_HUMAN |
 | Western blot analysis of extracts of HeLa cells, using NUCB2 antibody (CAB12642) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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