Cell Biology Antibodies 7

Anti-NUBP2 Antibody (CAB16476)

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SKU:
CAB16476
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NUBP2 Antibody
Antibody SKU:CAB16476
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 142-271 of human NUBP2 (NP_036357.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:LO2, HepG2, NIH/3T3, Mouse lung, Mouse brain, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 142-271 of human NUBP2 (NP_036357.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MATI EALR PYQP LGAL VVTT PQAV SVGD VRRE LTFC RKTG LRVM GIVE NMSG FTCP HCTE CTSV FSRG GGEE LAQL AGVP FLGS VPLD PALM RTLE EGHD FIQE FPGS PAFA ALTS IAQK ILDA TPAC LP
Gene ID:10101
Uniprot:Q9Y5Y2
Cellular Location:Cytoplasm, Nucleus, centriole, centrosome, cilium axoneme, cytoskeleton, microtubule organizing center
Calculated MW:28kDa
Observed MW:28kDa
Synonyms:NUBP2, CFD1, NBP 2, NUBP1
Background:This gene encodes an adenosine triphosphate (ATP) and metal-binding protein that is required for the assembly of cyotosolic iron-sulfur proteins. The encoded protein functions in a heterotetramer with nucleotide-binding protein 1 (NUBP1). Alternative splicing results in multiple transcript variants.
UniProt Protein Function:NUBP2: Component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for maturation of extramitochondrial Fe/S proteins. May bind and transfer a labile 4Fe-4S cluster to target apoproteins. Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP2/CFD1 subfamily.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cytoplasm; spindle pole centrosome; nucleus

Molecular Function:protein binding; metal ion binding; 4 iron, 4 sulfur cluster binding; nucleotide binding; ATP binding

Biological Process: iron-sulfur cluster assembly

NCBI Summary:This gene encodes an adenosine triphosphate (ATP) and metal-binding protein that is required for the assembly of cyotosolic iron-sulfur proteins. The encoded protein functions in a heterotetramer with nucleotide-binding protein 1 (NUBP1). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]
UniProt Code:Q9Y5Y2
NCBI GenInfo Identifier:548962168
NCBI Gene ID:10101
NCBI Accession:NP_001271431.1
UniProt Secondary Accession:Q9Y5Y2,Q9NWB2, D3DU80,
UniProt Related Accession:Q9Y5Y2
Molecular Weight:28,825 Da
NCBI Full Name:cytosolic Fe-S cluster assembly factor NUBP2 isoform 3
NCBI Synonym Full Names:nucleotide binding protein 2
NCBI Official Symbol:NUBP2  
NCBI Official Synonym Symbols:CFD1; NBP 2; NUBP1  
NCBI Protein Information:cytosolic Fe-S cluster assembly factor NUBP2; homolog of yeast cytosolic Fe-S cluster deficient 1; nucleotide binding protein 2 (MinD homolog, E. coli); C447E6.1 (nucleotide binding protein 1 (E.coli MinD like) )
UniProt Protein Name:Cytosolic Fe-S cluster assembly factor NUBP2
UniProt Synonym Protein Names:Nucleotide-binding protein 2; NBP 2
Protein Family:Cytosolic Fe-S cluster assembly factor
UniProt Gene Name:NUBP2  
UniProt Entry Name:NUBP2_HUMAN
Anti-NUBP2 Antibody (CAB16476)Western blot analysis of extracts of various cell lines, using NUBP2 antibody (CAB16476) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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