Cell Biology Antibodies 7
Anti-NUBP1 Antibody (CAB16403)
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- SKU:
- CAB16403
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-NUBP1 Antibody |
| Antibody SKU: | CAB16403 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 201-320 of human NUBP1 (NP_002475.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 201-320 of human NUBP1 (NP_002475.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PQEV SLQD VRKE INFC RKVK LPII GVVE NMSG FICP KCKK ESQI FPPT TGGA ELMC QDLE VPLL GRVP LDPL IGKN CDKG QSFF IDAP DSPA TLAY RSII QRIQ EFCN LHQS KEEN LISS |
| Gene ID: | 4682 |
| Uniprot: | P53384 |
| Cellular Location: | Cell projection, Cytoplasm, Nucleus, centriole, centrosome, cilium axoneme, cilium basal body, cytoskeleton, microtubule organizing center |
| Calculated MW: | 33kDa/34kDa |
| Observed MW: | 38kDa |
| Synonyms: | NUBP1, NBP, NBP1, NBP35 |
| Background: | NUBP1 is a member of the NUBP/MRP subfamily of ATP-binding proteins. |
| UniProt Protein Function: | NUBP1: Implicated in the regulation of centrosome duplication. Component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for maturation of extramitochondrial Fe/S proteins. May bind and transfer 2 labile 4Fe-4S clusters to target apoproteins. Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell development/differentiation Chromosomal Location of Human Ortholog: 16p13.13 Cellular Component: cytoplasm; cytosol; plasma membrane Molecular Function:iron-sulfur cluster binding; nucleotide binding; protein binding Biological Process: cell growth; cellular iron ion homeostasis; iron-sulfur cluster assembly |
| NCBI Summary: | NUBP1 is a member of the NUBP/MRP subfamily of ATP-binding proteins (Nakashima et al., 1999 [PubMed 10486206]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | P53384 |
| NCBI GenInfo Identifier: | 257050984 |
| NCBI Gene ID: | 4682 |
| NCBI Accession: | P53384.2 |
| UniProt Secondary Accession: | P53384,Q32M30, Q498A9, Q53FS7, |
| UniProt Related Accession: | P53384 |
| Molecular Weight: | 33,412 Da |
| NCBI Full Name: | Cytosolic Fe-S cluster assembly factor NUBP1 |
| NCBI Synonym Full Names: | nucleotide binding protein 1 |
| NCBI Official Symbol: | NUBP1 |
| NCBI Official Synonym Symbols: | NBP; NBP1; NBP35 |
| NCBI Protein Information: | cytosolic Fe-S cluster assembly factor NUBP1 |
| UniProt Protein Name: | Cytosolic Fe-S cluster assembly factor NUBP1 |
| UniProt Synonym Protein Names: | Nucleotide-binding protein 1 |
| Protein Family: | Cytosolic Fe-S cluster assembly factor |
| UniProt Gene Name: | NUBP1 |
| UniProt Entry Name: | NUBP1_HUMAN |
 | Western blot analysis of extracts of HepG2 cells, using NUBP1 antibody (CAB16403) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded mouse kidney using NUBP1 Rabbit pAb (CAB16403) at dilution of 1:100 (40x lens). |
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