Name: Anti-NTMT1 Antibody (CAB7098)
Brand: Cell Biology Antibodies 10
SKU: CAB7098
Availability: OutOfStock

Antibody Name:	Anti-NTMT1 Antibody
Antibody SKU:	CAB7098
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human NTMT1 (NP_054783.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, SKOV3, U-87MG, A-431, Raji, Mouse brain, Rat ovary

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human NTMT1 (NP_054783.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTSE VIED EKQF YSKA KTYW KQIP PTVD GMLG GYGH ISSI DINS SRKF LQRF LREG PNKT GTSC ALDC GAGI GRIT KRLL LPLF REVD MVDI TEDF LVQA KTYL GEEG KRVR NYFC CGLQ DFTP EPDS YDVI WIQW VIGH LTDQ HLAE FLRR CKGS LRPN GIIV IKDN MAQE GVIL DDVD SSVC RDLD VVRR IICS AGLS LLAE ERQE NLPD EIYH VYSF ALR
Gene ID:	28989
Uniprot:	Q9BV86
Cellular Location:	Nucleus
Calculated MW:	15kDa/25kDa
Observed MW:	25kDa

Synonyms:	NTMT1, AD-003, C9orf32, HOMT1A, METTL11A, NRMT, NRMT1, NTM1A
Background:

UniProt Protein Function:	METTL11A: Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]- Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di- methylation of Pro in the Pro-Pro-Lys motif. Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1. Belongs to the methyltransferase superfamily. NTM1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 2.1.1.244; Methyltransferase, protein N-term; Methyltransferase Chromosomal Location of Human Ortholog: 9q34.11 Cellular Component: nucleoplasm; cytoplasm; nucleus Molecular Function:protein methyltransferase activity Biological Process: N-terminal peptidyl-proline di-methylation; spindle organization and biogenesis; N-terminal peptidyl-alanine tri-methylation; chromosome segregation
NCBI Summary:	The METTL11A gene encodes an N-terminal methyltransferase for the RAN (MIM 601179) guanine nucleotide exchange factor regulator of chromosome condensation 1 (RCC1; MIM 179710). METTL11A enzyme alpha-N-methylates other protein targets such as SET (MIM 600960) and RB (MIM 180200).[supplied by OMIM, Nov 2010]
UniProt Code:	Q9BV86
NCBI GenInfo Identifier:	74761281
NCBI Gene ID:	28989
NCBI Accession:	Q9BV86.3
UniProt Secondary Accession:	Q9BV86,Q5SZB9, Q9UI28, A8K4J2, A8K8G7,
UniProt Related Accession:	Q9BV86
Molecular Weight:	15,896 Da
NCBI Full Name:	N-terminal Xaa-Pro-Lys N-methyltransferase 1
NCBI Synonym Full Names:	N-terminal Xaa-Pro-Lys N-methyltransferase 1
NCBI Official Symbol:	NTMT1
NCBI Official Synonym Symbols:	NRMT; NTM1A; AD-003; HOMT1A; C9orf32; METTL11A
NCBI Protein Information:	N-terminal Xaa-Pro-Lys N-methyltransferase 1; N-terminal RCC1 methyltransferase; X-Pro-Lys N-terminal protein methyltransferase 1A; alpha N-terminal protein methyltransferase 1A; methyltransferase-like protein 11A
UniProt Protein Name:	N-terminal Xaa-Pro-Lys N-methyltransferase 1
UniProt Synonym Protein Names:	Alpha N-terminal protein methyltransferase 1A; Methyltransferase-like protein 11A; N-terminal RCC1 methyltransferase; X-Pro-Lys N-terminal protein methyltransferase 1A; NTM1A
Protein Family:	N-terminal Xaa-Pro-Lys N-methyltransferase
UniProt Gene Name:	NTMT1
UniProt Entry Name:	NTM1A_HUMAN

	Western blot analysis of extracts of various cell lines, using NTMT1 antibody (CAB7098) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of A-549 cells using NTMT1 antibody (CAB7098). Blue: DAPI for nuclear staining.