Epigenetics & Nuclear Signaling Antibodies 4
Anti-NTHL1 Antibody (CAB6820)
(No reviews yet)
Write a Review
- SKU:
- CAB6820
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-NTHL1 Antibody |
| Antibody SKU: | CAB6820 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NTHL1 (NP_002519.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | A-549, HeLa, LO2, MCF-7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NTHL1 (NP_002519.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MCSP QESG MTAL SARM LTRS RSLG PGAG PRGC REEP GPLR RREA AAEA RKSH SPVK RPRK AQRL RVAY EGSD SEKG EGAE PLKV PVWE PQDW QQQL VNIR AMRN KKDA PVDH LGTE HCYD SSAP PKVR RYQV LLSL MLSS QTKD QVTA GAMQ RLRA RGLT VDSI LQTD DATL GKLI YPVG FWRS KVKY IKQT SAIL QQHY GGDI PASV AELV ALPG VGPK MAHL AMAV AWGT VSGI AVDT HVHR IANR LRWT KKAT KSPE ETRA ALEE WLPR ELWH EING |
| Gene ID: | 4913 |
| Uniprot: | P78549 |
| Cellular Location: | Mitochondrion, Nucleus |
| Calculated MW: | 32kDa/33kDa/34kDa |
| Observed MW: | 37kDa |
| Synonyms: | NTHL1, FAP3, NTH1, OCTS3, hNTH1 |
| Background: | The protein encoded by this gene is a DNA N-glycosylase of the endonuclease III family. Like a similar protein in E. coli, the encoded protein has DNA glycosylase activity on DNA substrates containing oxidized pyrimidine residues and has apurinic/apyrimidinic lyase activity. |
| UniProt Protein Function: | NTHL1: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions. Belongs to the Nth/MutY family. |
| UniProt Protein Details: | Protein type:DNA repair, damage; Mitochondrial; Lyase; EC 4.2.99.18; Hydrolase Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: mitochondrion; nucleoplasm; nucleus Molecular Function:4 iron, 4 sulfur cluster binding; DNA N-glycosylase activity; DNA-(apurinic or apyrimidinic site) lyase activity; double-stranded DNA binding; endonuclease activity; metal ion binding; oxidized purine base lesion DNA N-glycosylase activity; oxidized pyrimidine base lesion DNA N-glycosylase activity; protein binding Biological Process: base-excision repair, AP site formation; depyrimidination; nucleotide-excision repair, DNA incision, 5'-to lesion; positive regulation of defense response to virus by host Disease: Familial Adenomatous Polyposis 3 |
| NCBI Summary: | The protein encoded by this gene is a DNA N-glycosylase of the endonuclease III family. Like a similar protein in E. coli, the encoded protein has DNA glycosylase activity on DNA substrates containing oxidized pyrimidine residues and has apurinic/apyrimidinic lyase activity. [provided by RefSeq, Oct 2008] |
| UniProt Code: | P78549 |
| NCBI GenInfo Identifier: | 29840795 |
| NCBI Gene ID: | 4913 |
| NCBI Accession: | P78549.2 |
| UniProt Secondary Accession: | P78549,Q1MVR1, Q99566, Q99794, Q9BPX2, |
| UniProt Related Accession: | P78549 |
| Molecular Weight: | 32,839 Da |
| NCBI Full Name: | Endonuclease III-like protein 1 |
| NCBI Synonym Full Names: | nth-like DNA glycosylase 1 |
| NCBI Official Symbol: | NTHL1 |
| NCBI Official Synonym Symbols: | FAP3; NTH1; OCTS3; hNTH1 |
| NCBI Protein Information: | endonuclease III-like protein 1 |
| UniProt Protein Name: | Endonuclease III-like protein 1 |
| UniProt Synonym Protein Names: | Bifunctional DNA N-glycoslyase/DNA-(apurinic or apyrimidinic site) lyase |
| Protein Family: | Neutral trehalase |
| UniProt Gene Name: | NTHL1 |
| UniProt Entry Name: | NTH_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NTHL1 antibody (CAB6820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
View AllClose
Related Products
Human NTHL1 Recombinant Protein (RPPB2042)
Enzymes Recombinant Proteins
system_update_altDatasheetsystem_update_altMSDS
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीक
